MORPHOMETRIC VALUATION OF FEATURES OF STRUCTURAL RECONSTRUCTION OF HEMOMICROCIRCULATORY BED OF TONGUE AT DESQUAMATIVE GLOSSITIS

Hnatyuk M. S., Tatarchuk L. V., Bodnarchuk I. V.

MORPHOLOGY

Scentific article

Desquamative glossitis is a inflammatory and dystrophic affect of mucosa of the tongue, which is most common in diseases of the gastrointestinal tract and the hematopoietic system. Desquamative glossitis as an independent pathology is often found, and its diagnosis is not easy. The purpose of the research – morphometric study of changes in blood vessels of the hemomicrocirculatory bed at desquamative glossitis. Metods and material. The vessels of the hemomicrocirculatory bed of the tongue were examined morphometrically in 30 white male rats, which were divided into 2 groups. The first group consisted of 15 intact animals, 2 – 15 rats with desquamative glossitis. Desquamative glossitis was modeled by the creation of burns of tongue with acetic acid. Euthanasia of animals was carried out by bloodletting in conditions of thiopental anesthesia 2 weeks after from the beginning of the experiment. The hemicrocirculatory bed of the tongue was studied by injection of its vessels into the carcass-gelatinous mixture, which was injected through the common carotid artery. The diameters of the arterioles, the precapillaries, the hemocapillaries, the postcapillaries, the venules, the density of the microvessels per 1 mm² of the tissues of the tongue were measured. Quantitative indicators were processed statistically. Results and discussion. It was established that the quantitative morphological parameters of the vessels of the hemomycrocirculatory bed of the tongue at desquamative glossitis have changed significantly. The diameter of the arterioles of the tongue decreased by 12.3 %, the diameter of the precapillaries – by 11.3 %, the diameter of the hemocapillaries – by 10.6 % (p <0.001) compared with the control. The venous vessels of the hemomicrocirculatory bed of the tongue was expanded in the simulated experimental conditions. At desquamative glossitis, the diameter of the postcapillaries increased by 16.4 %, venules – by 14.3 %, the density of microvessels – by 8.5 % (p <0.001) indicating deterioration of the trophic support of the investigated organ. The results of the study suggest that desquamative glossitis leads to pronounced structural reconstruction of the vessels of the hemomicrocirculatory bed of the tongue, which is characterized by pronounced narrowing of the arterioles, precapillaries, hemocapillaries and the expansion of the postcapillaries and venules, decreasing of the density of the microvessels, venous pletora, hypoxia, dystrophy, necrobiosis of cells and tissues, cells of infiltration and sclerosis.

desquamative glossitis, hemomicrocirculatory bed, morphometry.

1. Herasymyuk IYe, Fedorovych OA. Morfofunktsionalni zminy ta prostorova kharakterystyka sudynnoho rusla yazyka pry yoho opikakh riznoho henezu v eksperymenti. Halytsʹkyy likarsʹkyy visnyk. 2013;20(1):26-9. [in Ukrainian].
2. Danilevskyi MF, Borysenko AV, Antonenko MU. Zakhvorjuvania slyzovoj obolonky porozhnyny rota. Kyev: Medicyna; 2010. 640 s. [in Ukrainian].
3. Sorochinnikov AG, Dorosievich AYe. Gistologichieskaya i mikroskopicheskaya tiekhnika. Moskva: Medicyna; 2007. 448 s. [in Russian].
4.  Avtadnilov GG. Osnovy kolichestvennoy patologicheskoy anatomii. Moskva: Medicyna; 2002. 240 s. [in Russian].
5. Tatarchuk LV, Hnatjuk MS. Morphometrychnyy analiz osoblyvostey strukturnoy perestrojky arterij klubovoj kyshky pry postresekcijnij portalnij hypertenzij. Zdobutky klinichnoj ta eksperymentalnoj medicyny. 2018;2:116-21. [in Ukrainian].
6. Lapach SN, Gubenko AV, Babich PN. Statistichieskiie metody v medico-biologicheskikh issledovaniyakh Excell. Kyev: Morion; 2001. 410 s. [in Russian].
7.  Reznicov OH. Zahalni etychni pryntsypy eksperymentiv na tvarynakh. Endokrynolohiia. 2003;8(1):142-5. [in Ukrainian].
8. Hearnden V, Lomas H, Macniel S. Diffusion studies of nanometer polymersomes across fissue engineered human oral mucosa. Pharm. Res. 2009;26(7):1718-28.
9. Bajbakov VM. Morfofunktsionalni zminy venoznoho rusla yak lanky drenaznoj systemy yaechka pry travmuvanni sudynnych anastomoziv simjanoho kanatyka v eksperymenti. Klinichna anatomija ta operatyvna khirurgija. 2011;10(4):32-5. [in Ukrainian].
10. Pronina OМ, Koptev MM, Bilash SМ, Yeroshenko GA. Response of hemomicrocirculatory bed of internal organs on various external factors exposure based on the morphological research data. Svit medytsyny ta biolohiyi. 2018;1(63):153-7.

«Bulletin of problems biology and medicine» Issue 2 Part 1 (150), 2019 year, 243-245 pages, index UDK 616.145.19-092-02.616-001.17

10.29254/2077-4214-2019-2-1-150-243-245