Bambuliak A. V., Kuzniak N. B., Perebyinis P. P., Tkachyk S. V

THE RESULTS OF THE STUDY OF BIOCOMPATIBILITY SAMPLES THAT CONTAINING MULTIPOTENT MESENCHYMAL STROMAL CELLS OF ADIPOSE TISSUE


About the author:

Bambuliak A. V., Kuzniak N. B., Perebyinis P. P., Tkachyk S. V

Heading:

DENTISTRY

Type of article:

Scentific article

Annotation:

Objective. Study of biocompatibility of samples of implantation material at cellular level with content of multipotent mesenchymal stromal cells of adipose tissue. Object and methods. As a source of cells, the subcutaneous tissue of 15 laboratory rats was used, which, under cultivation, causes the growth of fibroblastic and fibroblast-like elements. The biocompatibility study by cell culture method in vitro was carried out in accordance with the Work Instruction No. 04/2013 -.V.L. Results. The biocompatibility of tissue equivalents of bone tissue on the basis of MMSC-AT was characterized by an increase in the area of compact and mesh-shaped growth zones and the zone of migratory fibroblasts. At the 10th day of observation, the cell population of the investigated fragments entered the degeneration phase, which was manifested in significant vacuolization of the cytoplasm and its granular rebirth. Conclusions. Consequently, the tissue equivalent of bone tissue based on MMSC-AT can be candidates for regenerative medicine, and the study of their use in experimental animals gave an opportunity to broaden the understanding of the characteristics of MMSC-AT in order to optimize their subsequent clinical application and the implementation of new approaches in different directions of dentistry.

Tags:

multipotent mesenchymal stromal cells of adipose tissue, osteoplastic materials, biocompatibility

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Publication of the article:

«Bulletin of problems biology and medicine» Issue 2 Part 1 (150), 2019 year, 290-294 pages, index UDK 611.018.26:575.53

DOI: