Novikova O. Yu., Bondarenko T. P.

CHARACTERISTICS OF NATIVE AND CRYOPRESERVED CULTURE OF DERMAL PAPILLA OF NEWBORN RABBITS VIBRISSA


About the author:

Novikova O. Yu., Bondarenko T. P.

Heading:

BIOLOGY

Type of article:

Scentific article

Annotation:

The hair follicle is a complex dynamically changing formation formed by cells of different germ layers. Of particular interest is the pool of cells of the dermal papilla (DP), which are derivatives of the neural crest. The aim of the work was to study the effect of cryopreservation on the morphofunctional properties and growth potential of the culture of DP vibrissa DP cells in rabbits in native and cryopreserved cultures and in various cultivation methods, in a monolayer and in the form of multicellular spheroids (MS). The average size of the MS, measured on the 20th day after reseeding, was 370.00 ± 35.56; observations indicate that after this period the MS did not increase in size anymore and began to degrade. To study the effect of cryopreservation on the growth of MS, cryopreservation was performed in a cryoprotective medium based on DMEM with the addition of 10% DMSO. The average size of MS obtained from a suspension of cryopreserved culture, on the 20th day after reseeding, was 256.50 ± 33.00 (p = 0.05). Thus, there is a significant decrease in the size of MS formed from a cryopreserved culture, which is associated with a slowdown in cell aggregation and proliferation rate during the formation of MS. The proliferative potential of cells in the composition of 2D and 3 D-cultures was studied by counting AP on the 3rd day after reseeding. It was found that the proliferation coefficient in the monolayer was 5.44, while in the spheroids it was 4.27. Thus, there is probably a tendency toward a decrease in proliferation indices in the MS. Cryopreservation does not have a significant effect on the proliferation intensity in the rabbit DP culture – in the monolayer and in the MS. On the 15th day, the proliferative activity of the monolayer culture remained practically unchanged – 8.53, while in spheroids it increased almost 2 times compared to 7 days – 10.36. Thus, the activity of proliferative processes in MS is inferior to that in a monolayer culture at the initial stages of growth, however, due to the absence of contact inhibition, their growth takes longer

Tags:

dermal papilla, proliferation, multicellular spheroids, cryopreservation

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Publication of the article:

«Bulletin of problems biology and medicine» Issue 3 (152), 2019 year, 54-57 pages, index UDK 611.481.085

DOI: