THE DYNAMICS OF BEHAVIORAL TESTS IN RATS WITH INTRACEREBRAL HEMORRHAGE AFTER THE INJECTION OF CRYOPRESERVED NEURAL CELLS

Zolotko K. M., Sukach O. M., Kompaniets A. M.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Intracerebral hemorrhage is accompanied by severe complications and high mortality. Effective treatment of this disease does not currently exist. Cryopreserved neural cells therapy can be one such approach. The aim of the study was to investigate the effect of rat fetuses cryopreserved neural brain cells, as well as their aggregates injection on the rehabilitation of the animals’ motor activity after intracerebral hemorrhage. Object and methods of research. The experiments have been conducted on 33 white outbred male rats having weights from 250 to 350 grams. All animals were operated on to model intracerebral hemorrhage in the striatum. 1.5 x 106 cryopreserved neural cells as well as aggregates of neural cells were injected into the lateral ventricles of experimental animals. The rats of the 1-st control group were implanted with DMEM/F12 medium. The animals of the 2-d control group were implanted with destroyed cells. Functional impairment in rats after intracerebral hemorrhage was assessed by the results of the staircase test, the adhesive removal test and the corner tern test. Testing was carried out before the experiment, as well as after 1, 2, 7, 14, 21, 28, 35 and 42 days after the intracerebral hemorrhage modelling. The statistical significance of the obtained results was assessed by the Kruskal-Wallis and Mann-Whitney tests for pairwise comparisons at a significance level of 0.05. The median was used as the mean. Result and discussion. It was found that destroyed neural cells had a moderate positive effect for 2 days, which became apparent in the improvement in skilled reaching tasks. Neural cells had a short-term effect during the 1-st week in the recovery of subtle skills and tactile sensitivity. The aggregates of neural cells contributed to the restoration of subtle skills and tactile sensitivity during the first 7 days. After 21 days, the neural cells’ aggregates application was effective in reducing the asymmetry of sensory-motor deficit and the restoration of skilled reaching tasks. The cell implantation effectiveness can be explained by paracrine stimulating effects of the neural cells. The higher efficacy of the aggregates may be the result of their longer survival under unfavorable conditions of ischemia and inflammation compared to neural cells. Conclusions. It was shown that the implantation of cryopreserved neural cells’ aggregates to rats with intracerebral hemorrhage contributes to a more effective recovery of behavioral tests compared with a suspension of cryoconserved neural cells.

intracerebral hemorrhage, rats, cryopreserved, neural cells, aggregates, staircase test, corner turn test, adhesive removal test.

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«Bulletin of problems biology and medicine» Issue 3 (152), 2019 year, 108-112 pages, index UDK 616.831-005.1:615.361:611.018.82.013]:57.086.13

10.29254/2077-4214-2019-3-152-108-112