THE MODULATING EFFECT OF THE PESTICIDE LAMBDA-CYHALOTHRIN IN THE STUDY OF REPRODUCTIVE TOXICITY IN MALE AND FEMALE WISTAR HAN RATS

Kolianchuk Y. V., Shepelskaya N. R., Prodanchuk N. G., Bubalo N. N., Petrashenko G. I.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Lambda-cyhalothrin is one of the most widely used insecticides in the world. The purpose of this study was to identify the hazard of its reproductive toxicity for males and females Wistar Han rats and a comparative assessment of sexual sensitivity of the experimental animals to the test compound. Lambda-cyhalothrin (LCT) technical 95.6%, was administered daily by gavage in doses of 0.0; 0.3 and 3.0 mg/ kg body weight during 11 weeks for males and 10 weeks for females. After the scheduled exposure period, the treated animals mated with intact males and females. Functional indicators of gonad state and the animal’s ability to reproduction were examined after the end of exposure period. The duration and the frequency of each stage of the estrous cycle in female rats and the number of motile sperm, the total amount of sperm and the number of abnormal forms of germ cells of the male rats were studied. The reproductive function state in females was evaluated on day 20th of pregnancy. Thereby the number of corpora lutea in the ovaries, number of alive, dead and resorbed fetuses and embryos, the fetus weight, total weight of litters, the occurrence of malformations. Theindexes of mating, conception, fertility, pregnancy, and the length of precoital interval were taken into account. The studies were conducted in accordance with the recommendations of the Bioethics Commission and the standard operating procedures of the Center, within the Good Laboratory Practice (GLP) requirements. Analysis of the study’s results have shown that the maximum LCT dose causes systemic toxic effects on males and a modulating effect on the reproductive system of males and females, characterized by a decrease in body weight and impaired motility of sperm in males and a decrease of conception and fertility indexes and average weight of fetuses in females. Thus, the males were more sensitive to the systemic toxic effect of the test compound, and the females – to the damaging effect of this pesticide on the reproductive parameters. Within the studied range of doses in the experiment on the males and female of Wistan Han rats, no-observed effect level (NOEL) of LCT is the dose of 0.3 mg/kg of body weight; the low-observed effect level (LOEL) is 3 mg/kg of body weight.

reproductive toxicity, modulating effect, lambda-cyhalothrin, male and female Wistar Han rats

1. Tu W, Xu C, Lu B, Lin C, Wu Y, Liu W. Acute exposure to synthetic pyrethroids causes bioconcentration and disruption of the hypothalamus–pituitary–thyroid axis in zebrafish embryos. Science of the total environment. 2016;542:876-85.
2. Bownik A, Kowalczyk M, Bańczerowski J. Lambda-cyhalothrin affects swimming activity and physiological responses of Daphnia magna. Chemosphere. 2019;216:805-11.
3. Chang J, Xu P, Li W, Li J, Wang H. Enantioselective Elimination and Gonadal Disruption of Lambda-Cyhalothrin on Lizards (Eremias argus). Journal of agricultural and food chemistry. 2019;67(8):2183-9.
4. Ceuppens B, Eeraerts M, Vleugels T, Cnops G, Roldan-Ruiz I, Smagghe G. Effects of dietary lambda-cyhalothrin exposure on bumblebee survival, reproduction, and foraging behavior in laboratory and greenhouse. Journal of pest science. 2015;88(4):777-83.
5. Birolli WG, Arai MS, Nitschke M, Porto AL. The pyrethroid (±)-lambda-cyhalothrin enantioselective biodegradation by a bacterial consortium. Pesticide biochemistry and physiology. 2019;156:129-37.
6. Saillenfait AM, Ndiaye D, Sabaté JP. Pyrethroids: exposure and health effects–an update. International journal of hygiene and environmental health. 2015;218(3):281-92.
7. Campos E, Freire C. Exposure to non-persistent pesticides and thyroid function: a systematic review of epidemiological evidence. International journal of hygiene and environmental health. 2016;219(6):481-97.
8. Cooper DS, Biondi B. Subclinical thyroid disease. The Lancet. 2012;379(9821):1142-54. Available from: https://doi.org/10.1016/S0140- 6736(11)60276-6
9. Aguilar-Garduño C, Lacasaña M, Blanco-Muñoz J, Rodríguez-Barranco M, Hernández AF, Bassol S, et al. Changes in male hormone profile after occupational organophosphate exposure. A longitudinal study. Toxicology. 2013;307:55-65. Available from: https://doi.org/10.1016/j. tox.2012.11.001
10. Khan DA, Ahad K, Ansari WM, Khan H. Pesticide exposure and endocrine dysfunction in the cotton crop agricultural workers of southern Punjab, Pakistan. Asia Pacific Journal of Public Health. 2013;25(2):181-91. Available from: https://doi.org/10.1177/1010539511417422
11. Orton F, Rosivatz E, Scholze M, Kortenkamp A. Widely used pesticides with previously unknown endocrine activity revealed as in vitro antiandrogens. Environmental health perspectives. 2011;119(6):794-800.
12. Zhao M, Chen F, Wang C, Zhang Q, Gan J, Liu W. Integrative assessment of enantioselectivity in endocrine disruption and immunotoxicity of synthetic pyrethroids. Environmental pollution. 2010;158(5):1968-73. Available from: https://doi.org/10.1016/j.envpol.2009.10.027
13. Shepelska NR, Kolianchuk YaV. Porivnialnyi analiz riznykh metodolohichnykh pidkhodiv do identyfikatsii reproduktyvnoi toksychnosti pestytsydiv. Vistnyk problem biolohii i medytsyny. 2018;145(3):238-46. DOI: 10.29254/2077-4214-2018-3-145-238-246 [in Ukrainian].
14. Prodanchuk MH, Shepelska NR, Kolianchuk YaV. Neoborotnist antyandrohennoho efektu liambda-tsyhalotrynu pislia vidnovliuvalnoho periodu v doslidzhenni na samtsiakh shchuriv wistar han. Vistnyk problem biolohii i medytsyny. 2018;147(4):173-81. DOI: 10.29254/2077- 4214-2018-4-2-147-173-181 [in Ukrainian].
15. Guide for the care and use of laboratory animals. LAR Publication. National Academy Press. USA 1996. 140 p.
16. OECD Principles of Good Laboratory Practice. ENV/MC/CHEM(98)17. Environment Directorate Organisation for Economic Cooperation and Development. Paris 1998. 41 p.

«Bulletin of problems biology and medicine» Issue 3 (152), 2019 year, 127-131 pages, index UDK 615.9:632.95:612.6:591.16

10.29254/2077-4214-2019-3-152-127-131