SERUM LEVEL OF VASCULAR ENDOTHELIAL GROWTH FACTOR DURING PREGNANCY AND ALLELIC POLYMORPHISM OF rs2010963 VEGF GENE IN WOMEN WITH CONGENITAL HEART DEFECTS IN THE FETUS

Shargorodska E. B., Makukh G. V., Gaibonyuk I. E., Malakhova A. Y., Bobik O. B.

MEDICAL GENETICS

Scentific article

. The aim of the study was to determine the role of the maternal genotype of the rs2010963 (405С/G) loci of the VEGF gene and serum level of vascular endothelial growth factor as the risk factor of congenital heart defects in offspring. Object and Methods. The cases group consisted of 24 women who gave the fеtus with congenital heart defects. The control group included 45 women who gave birth to healthy children without a complicated genetic and obstetric history. The study used the polymerase chain reaction method, thermostable Taq polymerase oligonucleotide primers and restriction endonuclease (Thermo Fisher Scientific, USA). For the analysis of VEGF 405 C > G polymorphic loci, the RFLP method (restriction fragment length polymorphism) was used. To determine the serum concentration of vascular endothelial growth factor used a set of reagents “VEGF-ELISA-BEST”, designed to determine the concentration of VEGF in human serum by enzyme-linked immunosorbent assay. The results and discussion. According to the results of genetic testing and statistical calculations, the distribution of the genotypes and alleles of the polymorphic loci 405 C > G VEGF gene were compared with the control group. No significant association was found with the risk of CHD of the fetus according the distribution of the genotypes and alleles of the polymorphic loci 405 C > G VEGF gene (р=0.55). The serum level of vascular endothelial growth factor in pregnance women with genotype 405 GG VEGF was higher compare with another genotypes. It has been found that pregnant women with congenital heart defect in fetus have higher serum level of vascular endothelial growth factor compare with pregnant women without congenital abnormalities in fetus (p=0,029). Conclusions. The results indicate the prospect of further study of the level of endothelial growth factor in pregnant women at different stages of pregnancy, with the aim of further verification of this indicator as a marker of the congenital heart defect in fetus.

congenital heart defects, vascular endothelial growth factor.

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«Bulletin of problems biology and medicine» Issue 3 (152), 2019 year, 227-231 pages, index UDK 575.224.2+618.3+618.33:616.12-007-053.1+612.181

10.29254/2077-4214-2019-3-152-227-231