BIOLOGICAL PROPERTIES OF STAPHYLOCOCCI AND STREPTOCOCCI ISOLATED DURING INFLAMMATORY DISEASES OF THE RESPIRATORY TRACT

Skrypii K. Y., Holodok L. P., Skliar T. V.

MICROBIOLOGY

Scentific article

The aim of the work was to identify and study the biological properties of streptococci and staphylococci and their sensitivity to antibiotics for infections of the human respiratory tract. 266 clinical isolates were isolated, of which 171 strains belonged to S.aureus, 69 strains to S.pneumoniae, 26 – S.pyogenes. Identification of the species belonging to Staphylococcus spp., Streptococcus spp., was carried out by according to standard criteria: morphological, cultural, biochemical properties and pathogenicity factors. To establish the species belonging to staphylococci, a biological material was hung on the yellow-salt agar. Streptococci are generally grown on agar media supplemented with blood. This technique allows the detection of β-hemolysis, which is important for subsequent identification steps, and enhances the growth of streptococci by the addition of an external source of catalase. Used tests cultures for bacitracin susceptibility and PYR activity. For the identification of S.pneumoniae strains, used test on a catalase, oxidase activity, opacum sensitivity test, and lysis of bacterial culture in the presence of bile solubility test. The main etiological agent in diseases of the lower respiratory tract were S. pneumoniae – 80%. In inflammatory processes of the upper respiratory tract were bacteria S.aureus – 70%. There was a high level of occurrence of staphylococci and streptococci in children – 70%. Children can be considered as the main group of risk, in relation to the occurrence of staphylococci, streptococcus infections of the respiratory tract. It was established that the studied isolates of staphylococci showed high sensitivity to the antibiotics co-trimoxazole, amikacin, levofloxacin, oxacillin. The most sensitive strains of S.pyogenes, S.pneumoniae show antibiotics such as cefoxstine (88.4%), ciprofloxacin (84.6%), erythromycin, and clindamycin (80.7%). The research results can be applied in clinical practice for the treatment of infectious diseases of the respiratory tract. The obtained data testify to the need to study the conditionally pathogenic microflora in the development of diseases of the respiratory tract. It is necessary to continuously monitor their distribution and determine the sensitivity to antibacterial drugs.

opportunistic microflora, respiratory tract, staphylococci, streptococci, antibiotic resistance.

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«Bulletin of problems biology and medicine» Issue 3 (152), 2019 year, 273-277 pages, index UDK 579.61:616-078

10.29254/2077-4214-2019-3-152-273-277