DIFFERENTIAL DIAGNOSIS OF THE SEVERITY OF ABDOMINAL SEPSIS IN APPLYING THE METHOD OF PHASE MAPPING OF THE MICROSCOPIC IMAGES OF HISTOLOGICAL SECTIONS OF TISSUES OF INTERNAL ORGANS OF LABORATORY RATS

Solovay Yu. M., Solovay M. M.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Experimental studies were performed on 273 white nonlinear mature rats of both sexes weighing from 180 to 220 g. The animals were randomized to three experimental animals of 39 animals each and a control group of 39 animals. Experimental animals of groups I, II and III of abdominal sepsis were modeled according to their own methods. At the same time for modeling of varying severity used the introduction of 30%, 15%, and 7.5% of the freshly prepared solution of autocalcium. Experimentally tested optical location system photometrical mapping microscopic images of histological sections of internal organs of laboratory rats. The resulting album, maps of the distributions of the values of the phases of the points of digital microscopic images of histological sections of internal organs of rats of control group 1 and research groups 2 – 4 with varying severity of abdominal sepsis. Defined by a statistical reliability of differentiation of phase maps microscopic images of histological sections of internal organs of rats of control group 1 and research groups 2 – 4 with varying severity of septic disease. Found the most sensitive diagnostic statistical criteria for differentiation of phase maps microscopic images of histological sections of internal organs of rats of control group 1 and research groups 2 – 4 with varying severity of septic disease. Identified operational characteristics of the diagnostic power of the method of polarizing and phase microscopy of histological sections of tissues of internal organs of rats of control and experimental groups. Set balanced accuracy: differentiation of healthy and sick sepsis rats; intergroup differentiation severity of septic process the sick rats; intragroup differentiation in patients with the sepsis rats.

abdominal sepsis, the experiment, the method of phase mapping.

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«Bulletin of problems biology and medicine» Issue 3 (157), 2020 year, 149-157 pages, index UDK 616.381-002.3:616.94]-036.1-079.4

10.29254/2077-4214-2020-3-157-149-157