Pantus A. V., Rozhko M. M., Bagrii M. M., Kostyuk V. M., Yarmoshuk I. R.


About the author:

Pantus A. V., Rozhko M. M., Bagrii M. M., Kostyuk V. M., Yarmoshuk I. R.



Type of article:

Scentific article


Surgical interventions for various pathologies such as cysts of the jaws, chronic osteomyelitis and periodontitis often involve the use of osteoplastic materials to restore bone tissue. Their role is performed by a granular framework based on tricalcium phosphate and hydroxyapatite, but still the inability to restore a full-fledged bone structure remains a significant problem. In this regard, a new direction in reconstructive surgery has been formed – tissue engineering, the purpose of which is to restore biological functions, ie tissue regeneration, and not just its replacement with synthetic material. This approach allows you to purposefully manage the structural and functional state of cells involved in regenerative processes. The problem facing tissue engineering is to optimize the selection, reproduction and differentiation of cells, to construct matrices or delivery systems, contributing to the maintenance, coordination of tissue regeneration in three dimensions. Today in granular dental practice the role of matrices is performed by granular skeletons based on tricalcium phosphate and hydroxyapatite, but still the inability to restore a full-fledged bone structure remains a significant problem. One of the important criteria that must be taken into account when constructing a matrix is its ability to form an optimal substrate for substrate substrate. The purpose of the study is to experimentally assess the nature of the development of the microvasculature in all periods of subcutaneous implantation of a biopolymer fibrous matrix. The study was conducted on 50 laboratory animals (rabbits), which were divided into 2 groups. The first comparison group: 25 animals underwent surgery, which included the formation of a defect in the bone tissue. The second group: 25 animals developed a defect with subsequent implantation of a biopolymer matrix. Based on the histological studies, it was found that the formation of bone tissue occurred through the entire thickness of the fibrous polymer matrix in three mutually perpendicular directions. This fact is confirmed by the formation of a large proportion of osteoid in the early stages of regeneration. This in turn indicates a pronounced frame function of the polymeric microfiber matrix synthesized by us. That is, a group of polymer fibers creates a kind of substrate for the construction of tissues on it.


biopolymer, bioimplant, collagen fibers.


  1. Sharma A, Faubion WA, Dietz AB. Regenerative Materials for Surgical Reconstruction: Current Spectrum of Materials and a Proposed Method for Classification. Mayo Clin. Proc. 2019;94(10):2099-116.
  2. Conway J, Jacquemet G. Cell matrix adhesion in cell migration. Essays in Biochemistry. 2019;63(5):535-51.
  3. Mader JT, Calhoun J, Cobos J. In vitro evaluation of antibiotic diffusion from antibiotic-impregnated biodegradable beads and polymethylmethacrylate beads. Antimicrob. Agents Chemother. 1997;41(2):415-8.
  4. Markakis K, Faris AR, Sharaf H, Barzo Faris, Rees S, Bowling FL. Local Antibiotic Delivery Systems: Current and Future Applications for Diabetic Foot Infections. Int. J. Lower Extremity Wounds. 2018;17(1):14-21.
  5. Marson BA, Deshmukh SR, Grindlay DJC, Ollivere BJ, Scammell BE. A systematic review of local antibiotic devices used to improve wound healing following the surgical management of foot infections in diabetics. Bone Joint Journal. 2008;100-B(11):1409-15.
  6. Garvin K, Feschuk C. Polylactide-polyglycolide antibiotic implants. Clin. Orthop. Relat. Res. 2005;437:105-10
  7. Teupe C, Meffert R, Winckler S, Ritzerfeld W, Törmälä P, Brug E. Ciprofloxacin-impregnated poly-L-lactic acid drug carrier. New aspects of a resorbable drug delivery system in local antimicrobial treatment of bone infections. Arch. Orthop. Trauma. Surg. 1992;112(1):33-5.
  8. Suchy T, Supova M, Klapkova E, Adamkova V, Zavora J, Zaloudkova M, et. al. The release kinetics, antimicrobial activity and cytocompatibility of differently prepared collagen/hydroxyapatite/vancomycin layers: Microstructure vs. Nanostructure. Eur. J. Pharm. Sci. 2017;100:219-29.
  9. Nishimura J, Nakajima K, Souma Y, Takahashi T, Ikeguchi N, Takenaka R, et al. The possibility of using fibrin-based collagen as an antibiotic delivery system. Surg. Today. 2013;43(2):185-90.
  10. Costa Almeida CE, Reis L, Carvalho L, Costa Almeida CM. Collagen implant with gentamicin sulphate reduces surgical site infection in vascular surgery: a prospective cohort study. Int. J. Surg. 2014;12(10):1100-4.
  11. Rapetto F, Bruno VD, Guida G, Marsico R, Chivasso P, Zebele C. Gentamicin-Impregnated Collagen Sponge: Effectiveness in Preventing Sternal Wound Infection in High-Risk Cardiac Surgery. Drug Target Insights. 2016;10(1):9-13.
  12. Poriadok provedennia naukovymy ustanovamy doslidiv, eksperymentiv na tvarynakh. Ofitsiinyi visnyk Ukrainy. Ofits. vyd. 2012;24:82. [in Ukrainian].

Publication of the article:

«Bulletin of problems biology and medicine» Issue 3 (157), 2020 year, 241-245 pages, index UDK 616.314-083:528.315-38