THE DEGREE OF ACCUMULATION OF CADMIUM AND ZINC IN ADULT AND RAT EMBRYOS

Shamelashvili K. L.

BIOLOGY

Scentific article

Cadmium is a toxic heavy metal that is harmful to health. It has a pronounced tendency to accumulate in the body, including in the liver. Also has a toxic effect on embryo development. Able to compete for a place in biomolecules with zinc, which has the same degree of oxidation. The aim of the study was to determine the degree of accumulation of cadmium and zinc in the blood, liver of adult rats and in rat embryos against the background of exposure to cadmium in isolated administration and in combination with zinc succinate. Experimental studies were performed on female and male Wistar rats. For embryonic examination, female rats with a date of gestation were obtained using the method of vaginal swabs. On the 13th and 19th day of pregnancy, an operative slaughter was performed. To simulate the effects and toxic effects of exposure to cadmium chloride, we administered cadmium chloride solution (at a dose of 2.0 mg/kg) per day to females throughout pregnancy. In the second experimental group, a combined administration of cadmium chloride (at a dose of 2.0 mg/kg) and zinc succinate (at a dose of 5.0 mg/kg) was performed. Males were injected with test compounds in the same concentrations and at the same time as females. Slaughter was performed on the 13th and 19th day of administration of experimental compounds. Quantitative determination of cadmium and zinc was performed by atomic emission analysis on an EMAS-200 CCD spectrometer. The reliability of statistical studies was assessed using Student’s t-test. According to the results, it was found that the joint introduction of zinc and cadmium helps to reduce the concentration of cadmium in the liver, blood and embryos. The highest content of cadmium is found in the liver. Different degrees of zinc accumulation in the liver of males and pregnant females were revealed. It has also been experimentally determined that during pregnancy under adverse conditions (cadmium salt poisoning) the concentration of zinc in the liver increases. Cadmium and zinc are able to penetrate into rat embryos and accumulate there.

cadmium chloride, zinc succinate, embryogenesis, liver, blood.

1. Irfan M, Hayat S, Ahmad A, Alyemeni MN. Soil cadmium enrichment: Allocation and plant physiological manifestations. Saudi J Biol Sci. 2013;20(1):1-10.
2. Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol. 2014;7(2):60-72.
3. Genchi G, Sinicropi MS, Lauria G, Carocci A, Catalano A. The Effects of Cadmium Toxicity. Int J Environ Res Public Health. 2020;17(11):3782.
4. Patrick L. Toxic metals and antioxidants: part II. The role of antioxidants in arsenic and cadmium toxicity. Altern Med Rev. 2003;8(2):106-28.
5. Pabis K, Gundacker C, Giacconi R, Basso A, Costarelli L, Piacenza F, et al. Zinc supplementation can reduce accumulation of cadmium in aged metallothionein transgenic mice. Chemosphere. 2018;211:855-60.
6. Brzozowska A. Harmful elements and iron, zinc and copper: interactions in the animal and human organism. Part III. Cadmium. Rocz Panstw Zakl Hig. 1991;42(3):269-76.
7. Nasiadek M, Danilewicz M, Klimczak M, Stragierowicz J, Kilanowicz A. Subchronic Exposure to Cadmium Causes Persistent Changes in the Reproductive System in Female Wistar Rats. Oxid Med Cell Longev. 2019 Dec 17;2019:6490820. DOI: 10.1155/2019/6490820
8. Ostrovskaya SS. Prenatal’noye vozdeystviye kadmiya. Biomedical and biosocial anthropology. 2016;27:196-9. [in Russian].
9. Fedorenkо VI. Obhrontuvannya dopustymykh dobovykh doz svyntsyu i kadmiyu v dobovykh ratsionakh kharchuvannya. Profilaktychna medytsyna. 2019;24(1):73-80. [in Ukrainian].

«Bulletin of problems biology and medicine» Issue 4 (158), 2020 year, 84-87 pages, index UDK 599.323.4:591.436:591.3:546.48:616-092.9

10.29254/2077-4214-2020-4-158-84-87