CONNECTION OF PDGFB GENE POLYMORPHISM rs1800818 WITH SURGICAL TREATMENT RELAPSES OF DIABETIC MACULOPATHIA IN TYPE 2 DIABETES MELLITUS

Panchenko Iu. O

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

In diabetic eyes lesions, the importance of the Platelet-Derived Growth Factor (PDGF-BB), its genes and receptors has been shown. Aim of this study: to determine the association of the rs1800818 PDGFB gene with relapses in the surgical treatment of diabetic maculopathy (DMP). Object and methods: 313 patients with type 2 diabetes and 95 controls. Patients underwent a three-port closed subtotal vitrectomy, which, according to the testimony, was combined with peeling of the inner border membrane, panretinal laser retinal coagulation and cataract phacoemulsification. Blood levels before surgery were determined by PDGF-BB (R&D Systems; USA), rs1800818 – real-time polymerase chain reaction (TaqMan Mutation Detection Assays, Life-Technology, USA). Statistica 10 (StatSoft, Inc., USA) was used for statistical data processing. Results. The blood PDGF-BB content was significantly increased compared to the control. At DMP relapses PDGFBB before the beginning of surgical treatment was significantly higher than without relapses. The PDGFB gene rs1800818 polymorphism is associated with DMP: the minor allele T reduced the risk by 1.5-fold. The association of polymorphism with the severity of diabetic retinopathy and the complexes of surgical interventions were not found. rs1800818 has been associated with the surgical treatment relapses: the relapses risk in carriers of minor C allele has been significantly reduced. C-carriers had a lower PDGF-BB content than T-carriers, which had statistical significance for both patients and controls (p≤0.001) and could explain the protective effect of minor C allele for DMP relapses. Conclusion. The obtained results broaden the understanding of the DMP relapses development factors and substantiate the direction of targeted therapy – reduction of PDGF-BB blood content.

diabetic maculopathy, type 2 diabetes mellitus, PDGF-BB, rs1800818

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«Bulletin of problems biology and medicine» Issue 4 Part 1 (153), 2019 year, 138-142 pages, index UDK 617.735-002-02:616.633.66+616.155.2]-076.5

10.29254/2077-4214-2019-4-1-153-138-142