ANALYSIS OF THE POST-TRAUMATIC DYNAMICS OF DENSITY AND MINERAL COMPOSITION OF THE BONE TISSUE OF THE LOWER JAW OF THE RAT ON THE BACKGROUND OF THE DURABLE NALBUPHINUM INTAKE AND AFTER LINCOMYCINUM TREATMENT

Sohuyko R. R., Masna Z. Z., Pavliv H. I.

MORPHOLOGY

Scentific article

The results of numerous clinical and experimental studies show that the quality and mineral composition of bone tissue changes under the influence of various factors of both exogenous and endogenous origin. However, the data on changes that occur in bone tissue with long-term use of medications, in particular – of analgesic action, are fragmentary. The aim of our work was to study the peculiarities of the dynamics of the density and mineral composition of the bone tissue of the lower jaw of the rat after the application of bone-destructive trauma on the background of prolonged use of nalbuphinum with subsequent treatment with lincomycinum. Object and methods of research. The study was performed on 30 male rats weighing 180–200 g and 3.5 months of age. Opioid dependence was modeled in animals, on the background of bone-traumatic injury. After the injury, lincomycinum treatment was performed. Another 5 animals formed a control group. Injury was modeled by disruption of the integrity of the lower jaw bone tissue in the area of molars with the help of dental drill under thiopental anesthesia. Opioid dependence was modeled by intramuscular administration of narcotic analgesic nalbuphinum daily (1 time per day at the same time of day) according to the following schedule: 1st week – 8 mg/kg, 2nd week – 15 mg/kg, 3rd week – 20 mg/kg, 4th week – 25 mg/kg, 5th week – 30 mg/kg, 6th week – 35 mg/kg. Lincomycin (30% solution) was administered intraperitoneally at a dosage of 25 mg/kg once a day for 6 days after performing bone-destructive injury. Quality control of the bone tissue of the injured area of the mandible was performed using a dental radiovisiograph Siemens with Trophy Radiology software. The unit of measurement of tissue density was taken as the conventional units of grayness (CUG). Atomic absorption spectral analysis (AASA) was used to determine the mineral composition of the bone tissue of the lower jaw of the rat. The content of eight mineral elements was determined: calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na), potassium (K), iron (Fe), strontium (Sr), zinc (Zn). The concentration of the test elements was indicated in mg/g. Results. The data obtained from the study showed that, on the background of nalbuphinum intake, the density of the mandibular bone tissue increases for two weeks and decreases during the third week, returning, practically, to the control indices. That is why the end of the third week of the experiment was chosen as the term for traumatic injury. After trauma on the background of nalbuphinum intake and lincomycinum treatment, bone density increased sharply, exceeding the norm by 1.5 times, and decreased over the next two weeks, remaining slightly above control at the end of the sixth week of the experiment. The analysis of the mineral composition of the bone tissue of the mandible showed that during the first week of the experiment the content of calcium, phosphorus, iron, strontium and zinc increased, the content of sodium, magnesium and potassium decreased. At the end of the experiment, absolute values of calcium, sodium, iron and zinc content increased compared to the control, phosphorus and potassium decreased, and magnesium and strontium returned to normal. The analysis of the particle dynamics of the studied elements showed that in all bone samples among the four macroelements (Ca, P, Mg, Na), the largest specific share belongs to calcium, slightly less is the share of phosphorus, the smallest is the proportion of sodium and magnesium; among the microelements (K, Fe, Sr, Zn), the highest is the potassium content, and the lowest is the strontium and zinc specific shares. By the end of the experiment, the proportions of calcium, iron, strontium and zinc are increasing, phosphorus, sodium, magnesium and potassium are decreasing. Conclusions. Indicators of density and mineral composition of the bone tissue of the mandible of the rat after the application of bone-destructive injury against prolonged use of nalbuphinum with subsequent treatment with lincomycinum have pronounced dynamics, different and characteristic for each of the studied parameters. On the background of nalbuphinum intake, the bone density of the mandible increases for two weeks, and decreases during the third week, returning, practically, to the control indices. After bone traumatic injury and lincomycinum treatment, bone density increased sharply, exceeding the norm by 1.5 times and decreasing over the next two weeks, remaining slightly above control at the end of the sixth week of the experiment. The results of atomic absorption spectral analysis of the bone tissue of the lower jaw of the rat allow to determine the quantitative content of four macroelements (Ca, P, Mg, Na) and four microelements (K, Fe, Sr, Zn) and to study their dynamics during the experiment. The dynamics of the absolute indices of the content of the studied elements and their specific shares during the six weeks of the experiment are different. After six weeks of the experiment, absolute levels of calcium, sodium, iron, and zinc were increased compared to control, phosphorus and potassium were decreasing, and magnesium and strontium were returning to normal; the proportion of calcium, iron, strontium and zinc increase, phosphorus, sodium, magnesium and potassium – decrease.

bone tissue, nalbuphinum, density, mineral composition, bone-destructive trauma.

1. 1Adamovych OO, Masna ZZ, Henyk ID. Osoblyvosti vikovoyi dynamiky mineralnoho skladu kistkovoyi tkanyny v osib zriloho viku Prykarpatskoho rehionu. Ukrayinskyy morfolohichnyy almanakh. 2008;6(1):86-7. [in Ukrainian].
2. Adamovych OO. Osoblyvosti osteohennykh porushen pry eksperymentalnomu hipoatsydnomu stani shlunkovoyi sekretsiyi u vikovomu aspekti. Materialy KHIII Kongresu Svitovoyi Federatsiyi Ukrayinskykh Likarskykh Tovarystv. 2010 Ver 30 – Zhov 03; Lviv. Lviv: Lviv. Nats. med. un-t imeni Lanyla Halytskoho; 2010. s. 660. [in Ukrainian].
3. Adamovych OO, Masna ZZ, Safonova OV, Safonov AS. Zakonomirnosti vikovoyi dynamiky strukturnoyi perebudovy kistkovoyi tkanyny u shchuriv. Ukrayinskyy morfolohichnyy almanakh. 2010;8(3):214-5. [in Ukrainian].
4. Adamovych OO, Zayachkivska OS, Hzhehotskyy MR. Hipoatsydnist shlunkovoyi sekretsiyi yak modulyator porushen kistkovoyi mineralnoyi shchilnosti. Visnyk problem biolohiyi i medytsyny. 2011;1(2):98-9. [in Ukrainian].
5. Kordyyak OY, Masna ZZ, Hryshchuk HV. Otsenka myneralnoho sostava kostnoy tkany alveolyarnoho otrostka nyzhney chelyusty krys pry éksperymentalnom parodontyte. Zdravookhranenye. 2014;6:129-33. [in Russian].
6. Korenkov OV. Vplyv pryrodnoho hidroksylapatytu i b-trykaltsiyfosfatu na dynamiku zmin mekhanichnykh vlastyvostey v eksperymentalnomu defekti kompaktnoyi kistkovoyi tkanyny. Ortopedyya, travmatolohyya y protezyrovanye. 2017;1:14-20. DOI: http://dx.doi.org/10.15674/0030- 598720171 [in Ukrainian].
7. Safonov AS, Urbanovych AM, Safonova OV, Adamovych OO. Osteoporoz na foni hiperprolaktynemiyi. Materialy nauk.-prakt. konf. z mizhnar. uchastyu Dosyahnennya ta perspektyvy eksperymentalnoyi ta klinichnoyi endokrynolohiyi; 2010 Ber 1-2; Kharkiv. Kharkiv: Kharkivskyy nats. med. un-t; 2010. s. 140. [in Ukrainian].
8. Safonov AS, Safonova OV. Osteoporoz pry tsukrovomu diabeti. Materialy I konhresu Asotsiatsiyi endokrynolohiv Ukrayiny; 2010 Kvit 21-23; Kyyiv. Endokrynolohiya. 2010;15:59. [in Ukrainian].
9. Safonov AS, Safonova OV, Adamovych OO. Zakonomirnosti vikovoyi dynamiky strukturnoyi perebudovy kistkovoyi tkanyny u shchuriv. Materialy vseukr. nauk.-prakt. konf. Fiziolohichna ta reparatyvna reheneratsiya kistky: suchasnyy stan pytannya; 2010 Ver 23-24; Yevpatoriya. Ukrayinskyy morfolohichnyy almanakh. 2010;8(3):214-5. [in Ukrainian].
10. Ryzhuk K, Dahno L, Chaykovska S, Pavliv K. Peculiarities of structural reconstruction and mineral content dynamic of hard tissues of dentomandibular area in age aspect. Book of Abstracts The 5th International Symposium of Clinical and Applied Anatomy; 2013 May 24-26; Graz, Austria. Rev Arg de Anat Clin. 2013;5(2):140.
11. Yoshioka Y, Yamachika E, Nakanishi M, Ninomiya T, Nakatsuji K, Kobayashi Y, et al. Cathepsin K inhibitor causes changes in crystallinity and crystal structure of newly-formed mandibular bone in rats. Br J Oral Maxillofac Surg. 2018;56(8):732-8. DOI: 10.1016/j.bjoms.2018.08.003
12. Yoshioka Y, Yamachika E, Nakanishi M, Ninomiya T, Nakatsuji K, Matsubara M, et al. Molecular alterations of newly formed mandibular bone caused by zoledronate. Int J Oral Maxillofac Surg. 2018;47(9):1206-13. DOI: 10.1016/j.ijom.2018.02.002
13. Zayachkivska O, Kordiyak O, Adamovych O, Safonov A. Association between osteoporosis and no-related metabolic disorders in rats. Book of Abstracts The 5th International Symposium of Clinical and Applied Anatomy; 2013 May 24-26; Graz, Austria. Rev Arg de Anat Clin. 2013;5(2):97.
14. Korenkov OV. Kompyuterno-tomohrafichna otsinka zahoyennya defektu dovhoyi kistky u shchuriv pislya implantatsiyi v yoho porozhnynu osteoplastychnoho materialu na osnovi b-trykaltsiyfosfatu. Ortopedyya, travmatolohyya y protezyrovanye. 2014;3:5-9. DOI: http://dx.doi. org/10.15674/0030-5987201435-9 [in Ukrainian].
15. Korenkov OV. Kompyuterno-tomohrafichnyy i densytometrychnyy analiz vplyvu kaltsiy-fosfatnoyi biokeramiky na reheneratsiyu eksperymentalnoho defektu kompaktnoyi kistkovoyi tkanyny. Materialy nauk.-prakt. konf. z mizhnar. uchastyu Teoriya ta praktyka suchasnoyi morfolohiyi; 2016 Zhov: 5-7; Dnipro. Dnipro: Dnipropetrovska med. akademiya; 2016. s. 81-3. [in Ukrainian].
16. Masna ZZ, Harasym KhI, Krynytskyy RP, Dakhno LO. Vykorystannya mozhlyvostey radioviziohrafiyi dlya diahnostyky stanu kistkovoyi tkanyny. Materialy nauk. konhresu IV Mizhnarodni Pyrohovski chytannya, V z`yizdu anatomiv, histolohiv, embriolohiv i topohrafoanatomiv Ukrayiny; 2010 Cher 2-5; Vinnytsya. Vinnytsya: Vinnytskyy nats. med. un-t; 2010. s. 76. [in Ukrainian].
17. Cherkes MB. Vykorystannya promenevykh metodiv doslidzhennya dlya vyvchennya osoblyvostey perebudovy shchelepno-lytsevoyi dilyanky u vikovomu aspekti. Materialy KHV konhresu Svitovoyi federatsiyi Ukrayinskykh likarskykh tovarystv; 2014 Zhov 16-18; Chernivtsi. Chernivtsi – Kyyiv – Chikaho; 2014. s. 411. [in Ukrainian].
18. Masna ZZ, Dakhno LO. Zastosuvannya promenevykh metodiv pry vyvchenni anatomichnykh osoblyvostey shchelepno-lytsevoyi dilyanky. Ukrayinskyy morfolohichnyy almanakh. 2010;8(3):203. [in Ukrainian].
19. Masna-Chala OZ, Masna ZZ, Dakhno LO. Vyvchennya anatomichnykh osoblyvostey shchelepno-lytsevoyi dilyanky v tsyfrovomu renthenivskomu zobrazhenni. Ukrayinskyy morfolohichnyy almanakh. 2011;9(3):118-9. [in Ukrainian].
20. Korenkov OV. Reheneratsiya dovhoyi kistky pislya implantatsiyi v yiyi defekt b-trykaltsiyfosfatu. Ortopedyya, travmatolohyya y protezyrovanye. 2015;1:21-4. DOI: http://dx.doi.org/10.15674/0030-59872015121-24 [in Ukrainian].
21. Korenkov AV. Regeneration of the long bone after implantation into its defect of osteoplastic material «Cerabone®». Osteologicky Bulletin. 2016;21(1):24-7.
22. Sohuyko R, Masna Z. Analiz dynamiky mineralnoho skladu kistkovoyi tkanyny na tli opioyidnoho vplyvu. Pratsi naukovoho tovarystva im. Shevchenka. Medychni nauky. 2019;55(1):40-55. DOI: 10.25040/ntsh2019.01.04 [in Ukrainian].
23. Korenkov OV. Computed tomography densitometry of femoral defect healing after implantation of calcium phosphate bioceramics in rats. Bulgarian Journal of Veterinary Medicine. 2016;19(2):87-95. DOI: 10.15547 / bjvm.925
24. Poghosyan YM, Hakobyan KA, Poghosyan AY, Avetisyan EK. Surgical treatment of jaw osteonecrosis in «Krokodil» drug addicted patients. J Craniomaxillofac Surg. 2014;42(8):1639-43. DOI: 10.1016/j.jcms.2014.05.005 25. Zandi M, Dehghan A, Amini P, Rezaeian L, Doulati S. Evaluation of mandibular fracture healing in rats under zoledronate therapy: A histologic study. Injury. 2017;48(12):2683-7. DOI: 10.1016/j.injury.2017.10.026

«Bulletin of problems biology and medicine» Issue 4 Part 1 (153), 2019 year, 231-237 pages, index UDK 611.716.4-001-018.4:612.015.31:615.212.7:615.33]-08

10.29254/2077-4214-2019-4-1-153-231-237