Miroshnik D. B., Shckorbatov Y. G.

IMPACT OF DOXORUBICIN AND MAGNETIC FIELD ON VIABILITY OF EXFOLIATED HUMAN BUCCAL EPITHELIAL CELLS AND CHROMATIN STATE


About the author:

Miroshnik D. B., Shckorbatov Y. G.

Heading:

CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scentific article

Annotation:

At present it is not possible to achieve a complete efficacy in the treatment of doxorubicin because medication is administered before the manifestation of side effects. For this reason, many studies are being conducted to develop techniques to improve the effectiveness of doxorubicin treatment. It was proposed, for example, to use the combined effect of magnetic or electromagnetic fields and doxorubicin to reduce toxicological load on the human organism. The purpose of the present study was to investigate the combined effect of a static and rotating magnetic fields and doxorubicin on human buccal epithelial cells, and to determine the difference in the influence of a static magnetic field and a rotating one. The combined effect of doxorubicin and magnetic field of induction of 16.7 mT on the viability of isolated buccal epithelial cells of three donors was investigated. The magnetic field was used as a static if magnet was unmovable, and rotating magnetic field if magnet turned around at a speed of 500 rpm. Doxorubicin at a concentration of 2 μg/ml added to cell culture medium for 2 hours has been shown to increase the decrease of cell viability and to the heterochromatization of chromatin in cell nuclei. The influence of a static magnetic field of 16.7 mT for 10 minutes does not lead to a reliable change of the studied parameters (neither increase of heterochromatin granules nor increase of membrane permeability to ethidium bromide). The influence of the rotating magnetic field at the same time as doxorubicin leads to a decrease in the number of heterochromatin granules in the nuclei of the cells of all three donors and in a decrease in the permeability of the membranes to the ethidium bromide in the two donors compared to the variant effect of the doxorubicin alone. This indicates a protective effect of magnetic field in the case of using in combination with doxorubicin. The cause of the effect in the experiment is assumed to be connected with hormesis effect.

Tags:

cell viability, heterochromatin, cell membrane, membrane permeability, magnetic field.

Bibliography:

  1. Agudelo D, Bourassa P, Bérubé G, Tajmir-Riahi HA. Intercalation of antitumor drug doxorubicin and its analogue by DNA duplex: structural features and biological implications. International journal of biological macromolecules. 2014 May 1;66:144-50.
  2. Yang F, Chen H, Liu Y, Yin K, Wang Y, Li X, et al. Doxorubicin caused apoptosis of mesenchymal stem cells via p38, JNK and p53 pathway. Cellular Physiology and Biochemistry. 2013;32(4):1072-82.
  3. Alves AC, Magarkar A, Horta M, Lima JL, Bunker A, Nunes C, Reis S. Influence of doxorubicin on model cell membrane properties: insights from in vitro and in silico studies. Scientific reports. 2017 Jul 24;7(1):6343.
  4. Yang F, Teves SS, Kemp CJ, Henikoff S. Doxorubicin, DNA torsion, and chromatin dynamics. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer. 2014 Jan 1;1845(1):84-9.
  5. DBLTM DOXORUBICIN HYDROCHLORIDE. Data Sheet – New Zealand [Internet]. Hospira NZ Limited, 23 Haining Street, Te Aro, Wellington, New Zealand. Date of preparation 2012 April 18. Available from: [updated 2012 Apr 18; cited 2019 Nov 20]. Available from: https://www.google. com.ua/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&ved=0ahUKEwinrLbnh_3MAhUkEpoKHalYCbkQFghAMAQ&url=http%3A%2F%2Fw ww.medsafe.govt.nz%2Fprofs%2Fdatasheet%2Fd%2FDoxorubicinhydrochlorideinjmp.pdf&usg=AFQjCNETZvTfYZOJydGaUM3VC7KbVNMotA &bvm=bv.123325700,d.bGs&cad=rja
  6. Jeyaseelan R, Poizat C, Wu HY, Kedes L. Molecular mechanisms of doxorubicin-induced cardiomyopathy. Selective suppression of Reiske ironsulfur protein, ADP/ATP translocase, and phosphofructokinase genes is associated with ATP depletion in rat cardiomyocytes. Journal Biol. 1997;272(9):5828-32.
  7. Llach A, Mazevet M, Mateo P, Villejouvert O, Ridoux A, Rucker-Martin C, et al. Progression of excitation-contraction coupling defects in doxorubicin cardiotoxicity. Journal of molecular and cellular cardiology. 2019 Jan 1;126:129-39.
  8. Ansar SM, Jiang W, Mudalige T. Direct quantification of unencapsulated doxorubicin in liposomal doxorubicin formulations using capillary electrophoresis. International journal of pharmaceutics. 2018 Oct 5;549(1-2):109-14.
  9. Singal PK, Siveski-Iliskovic N, Hill M, Thomas TP, Li T. Combination therapy with probucol prevents adriamycin-induced cardiomyopathy. J Mol Cell Cardiol. 1995;27:1055-63.
  10. Green D, Bensely D, Schein P. Preclinical evaluation of WR-151327: an orally active chemotherapy protector. Cancer Res. 1994;54:738-41.
  11. Seifert CF, Nesser ME, Thompson DF. Dexrazoxane in the prevention of doxorubicin-induced cardiotoxicity. Ann Pharmacother. 1994;28:1063- 72. [Erratum, Ann Pharmacother 1994;28:1413].
  12. Williams GA, Johnson JR, Burke G. FDA oncology drugs advisory committee review of Zinecard (dexrazoxane, ADR-529, ICRF-187). Rockville. Md.: Center for Drug Evaluation and Research; 1992. р. 1-13.
  13. Hannan CJ Jr, Liang Y, Allison JD, Pantazis CG, Searle JR. Chemotherapy of human carcinoma xenografts during pulsed magnetic field exposure. Anticancer Research. 1994;14(4A):1521-24.
  14. Verdom BH, Abdolmaleki P, Behmanesh M. The static magnetic field remotely boosts the efficiency of doxorubicin through modulating ROS behaviors. Scientific reports. 2018;8(1):990.
  15. Ha PT, Le TTH, Bui TQ, Pham HN, Ho AS, Nguyen LT. Doxorubicin release by magnetic inductive heating and in vivo hyperthermia-chemotherapy combined cancer treatment of multifunctional magnetic nanoparticles. New Journal of Chemistry. 2019;43(14):5404-13.
  16. Verdom BH, Abdolmaleki P, Behmanesh M. The static magnetic field remotely boosts the efficiency of doxorubicin through modulating ROS behaviors. Scientific reports. 2018;8(1):990.
  17. Sabo J, Mirossay L, Horovcak L, Sarissky M, Mirossay A, Mojzis J. Effects of static magnetic field on human leukemic cell line HL-60. Bioelectrochemistry. 2002;56:227-31.
  18. Shckorbatov YG, Zhuravlyova LA, Navrotskaya VV, Miroshnichenko EV, Montvid PY, Shakhbazov VG, et al. Chromatin structure and the state of human organism. Cell biology international. 2005 Jan;29(1):77-81.
  19. Boltz RD, Fischer PA, Wicker LS, Peterson LB, Single UV. Excitation of Hoechst 33342 and ethidium bromide for simultaneous cell cycle analysis and viability determinations on in vitro cultures of murine B lymphocytes. Cytometry. The Journal of the International Society for Analytical Cytology. 1994 Jan 1;15(1):28-34.
  20. Baureus Koch CLM, Sommarin M, Persson BRR, Salford LG, Eberhardt JL. Interaction between weak low frequency magnetic fields and cell membranes. Bioelectromagnetics. 2003;24:395-402.
  21. Blackman CF, Blanchard JP, Benane SG, House DE. Empirical test of an ion parametric resonance model for magnetic field interactions with PC-12 cells. Bioelectromagnetics. 1994;15:239-60.

Publication of the article:

«Bulletin of problems biology and medicine» Issue 4 Part 2 (154), 2019 year, 157-161 pages, index UDK 576.32/.36:612.014

DOI: