COMPARATIVE CHARACTERISTICS OF THE RATE OF RELEASE OF ANTIBIOTICS FROM MICROFIBER POLYMER AND COLLAGEN MATRICES, TAKING INTO ACCOUNT THEIR STORAGE CONDITIONS

Pantus A. V.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

To date, a new trend is being pursued in medicine, which involves the combination of fibrous materials with therapeutic agents such as drug delivery systems and living cellsIn this regard, reconstructive surgery has formed a new direction – tissue engineering, which aims at restoring biological functions, that is, regenerating tissue, not just replacing it with synthetic material. This approach allows purposeful management of the structural and functional state of cells involved in regenerative processes. In addition, toxic solvents for polymers are used in the synthesis of micro and nanofibers in this way. At present, a cheaper and safer method for the synthesis of fibrous matrices and the use of antibiotic-impregnated such matrix implants in surgical dentistry remains relevant. The purpose of the study is to study the antibiotic-sorption capacity of the three-dimensional non-woven matrices we have created for the reconstruction of bone tissue defects made from polycaprolactone; the possibility of releasing an impregnated antibiotic from the matrix material. Samples of three-dimensional microfibrous nonwoven matrices for reconstruction of bone defects made according to our polycaprolactone technique were used. The microfiber frame we manufactured and the collagen pads were separated into fragments and sterilized by γ radiation. The impregnation of matrix samples was carried out under aseptic conditions by applying micropipette solutions of antibiotics (cefazolin at a final dose of 30 μg and lincomycin – 10 μg), followed by drying in a fat-free cabinet at a temperature of not more than 300C. To evaluate the preservation of antibiotics in samples of matrix materials and the possibility of their release in the active state in the environment used the most accessible and sufficiently sensitive biological test. The results obtained indicate that in the tested parameters the level of light and temperature did not significantly affect the activity dynamics of both antibiotics impregnated in both polycaprone and collagen matrices. In the analysis of the effect of temperature and light and time of storage of the samples by the method of two-factor analysis of variance reliable dependence of the activity of antibiotics is established only on the time of their storage. Pathogenic and conditionally pathogenic oral microflora are mainly streptococci, staphylococci and actinomycetes, which are mainly characterized by high sensitivity to cephalosporins in lincosamides (which led to the choice of antimicrobials for this development).

matrix materials, collagen, polycaprolactone, antibiotic impregnation, cefazolin, lincomycin

1. Sharma A, Faubion WA, Dietz AB. Regenerative Materials for Surgical Reconstruction: Current Spectrum of Materials and a Proposed Method for Classification. Mayo Clin. Proc. 2019;10(94):2099-116.
2. Markakis K, Faris AR, Sharaf H, Barzo F, Rees S, Bowling FL. Local Antibiotic Delivery Systems: Current and Future Applications for Diabetic Foot Infections. Int. J. Lower Extremity Wounds. 2018;1(17):14-21.
3. Conway J, Jacquemet G. Cell matrix adhesion in cell migration. Essays in Biochemistry. 2019;5(63):2012-9.
4. Marson BA, Deshmukh SR, Grindlay DC, Ollivere BJ, Scammell BE. A systematic review of local antibiotic devices used to improve wound healing following the surgical management of foot infections in diabetics. Bone Joint J. 2008;11(100):1409-15.
5. Teupe C, Meffert R, Winckler S, Ritzerfeld W, Törmälä P, Brug E. Ciprofloxacin-impregnated poly-L-lactic acid drug carrier. New aspects of a resorbable drug delivery system in local antimicrobial treatment of bone infections. Arch. Orthop. Trauma. Surg. 1992;1(112):33-5.
6. Nishimura J, Nakajima K, Souma Y. The possibility of using fibrin-based collagen as an antibiotic delivery system. Surg. Today. 2013;2(43):185- 90.
7. Mader JT, Calhoun J, Cobos J. In vitro evaluation of antibiotic diffusion from antibiotic-impregnated biodegradable beads and polymethylmethacrylate beads. Antimicrob. Agents Chemother. 1997;2(41):415-8.
8. Costa Almeida CE, Reis L, Carvalho L, Costa Almeida CM. Collagen implant with gentamicin sulphate reduces surgical site infection in vascular surgery: A prospective cohort study. Int. J. Surg. 2014;10(12):1100-4.
9. Garvin K, Feschuk C. Polylactide-polyglycolide antibiotic implants. Clin. Orthop. Relat. Res. 2005;435:105-10.

«Bulletin of problems biology and medicine» Issue 4 Part 2 (154), 2019 year, 172-177 pages, index UDK 616.314-083:528.315-38

10.29254/2077-4214-2019-4-2-154-172-177