THE STUDY OF ASSOCIATION BETWEEN HOTAIR GENE rs1899663 POLYMORPHISM AND METASTASIS DEVELOPMENT IN PROSTATE CANCER PATIENTS
About the author:
Volkogon A. D.
Type of article:
HOTAIR (HOX antisense intergenic RNA) is involved in regulation of many target genes expression at both epigenetic and transcriptional levels. It is known that HOTAIR leads to tumors development by methylation of Lys-27 residues (H3K27) and demethylation of Lys-4 residues (H3K4) of H3 histones of genes involved in cell division. It has been demonstrated that prostate cancer (PC) progression is associated with significant increase of HOTAIR transcripts number in malignant prostate cells. In addition, it is known that HOTAIR overexpression stimulates the PC cells proliferation, and HOTAIR knockdown leads to division suppression of malignant prostate cells. The aim of the study was to study of the possible association between HOTAIR gene rs1899663 locus and metastasis development in Ukrainian prostate cancer patients. Object and methods. The venous blood of PC 184 patients (80 with metastasis and 104 without metastatic signs) was used in current case-control study. Genotyping of participants by HOTAIR gene rs1899663-site was carried out by polymerase chain reaction followed by restriction fragment length polymorphism analysis (PCR-RFLP). Statistical analysis of obtained data was performed using SPSS 17.0 software package (Chicago, IL, USA). P values < 0.05 were considered as statistically significant. Results. It was revealed that frequency of GG-, GT-, and TT-genotypes (HOTAIR gene rs1899663 site) did not differ significantly between PC patients without metastases and with metastatic process signs (P = 0.291). Statistically significant association between different rs1899663-allelic variants and metastasis development in PC patients was not found both before and after adjusting for age, body mass index, smoking, and alcohol abuse (P > 0.05). Conclusion. Thus, this study results showed that HOTAIR gene rs1899663 polymorphic locus is not involved in metastasis occurrence in Ukrainian men with prostate cancer.
long non-coding RNA, HOTAIR, nucleotide polymorphism, metastasis, prostate cancer.
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Publication of the article:
«Bulletin of problems biology and medicine» Issue 4 Part 2 (154), 2019 year, 259-262 pages, index UDK 616.6-006:577.213/.216