Mirzebasov M. A.

THE STATE OF COLUMNAR EPITHELIAL CELLS OF THE VILLI OF THE DUODENAL MUCOSA OF RATS UNDER PROLONGED EXPOSURE TO EPICHLOROHYDRIN


About the author:

Mirzebasov M. A.

Heading:

MORPHOLOGY

Type of article:

Scentific article

Annotation:

Diseases of the digestive system in the modern world remain one of the serious problems. It is known that the state of the digestive system is largely dependent on the environment. The influence of anthropogenic pollutants can lead to the development of disorders of the digestive system. One of the most widespread pollutants is epichlorohydrin (ECH). This chemical can negatively affect human health. However, the regularities of the effects of ECH on the state of the duodenum have not been studied enough. There is also insufficient scientific data on the experimental justification of methods for preventing the development of disorders arising in duodenum due to the negative effects of ECH, and methods for correcting these disorders. The aim of the study was to assess the state of the columnar epithelial cells of the villi of the mucous membrane (MM), duodenum in terms of the long-acting ECH in rats, and determine the feasibility of application of an extract of Echinacea purpurea (EP) and thiotriazoline for the correction of occurring disorders. Under the influence of ECH, changes occurred in the nucleus and cytoplasm of columnar epithelial cells of villi of duodenum MM. Compared with the corresponding indicators of the control group rats, the cut-off area (Sca) of the nucleus and the Ph of the cytoplasm of these cells decreased, and the nuclear-cytoplasmic index (NCI) increased. The use of EP extract on the background of the introduction of ECH reduced the timing of reduction of Sca of the nucleus of columnar epithelial cells of villi caused by the action of ECH. The use of thiotriazoline during inhalation significantly reduced the time to reduce the Sca of the nucleus and the Sca of the cytoplasm of columnar epithelial cells of the villi. These facts indicate the expediency of using EP extract and thiotriazoline as correctors of duodenum MM disorders induced by ECH.

Tags:

duodenum, columnar epithelial cells, epichlorohydrin, rats

Bibliography:

  1. Sipponen P, Maaroos HI. Chronic gastritis. Scand J Gastroenterol. 2015;50(6):657-67.
  2. Minalyan A, Benhammou JN, Artashesyan A, Lewis MS, Pisegna JR. Autoimmune atrophic gastritis: current perspectives. Clin Exp Gastroenterol. 2017;10:19-27.
  3. Zhang M, Li Y. Eosinophilic gastroenteritis: A state-of-the-art review. J Gastroenterol Hepatol. 2017;32(1):64-72.
  4. Zagari RM, Rabitti S, Greenwood DC, Eusebi LH, Vestito A, Bazzoli F. Systematic review with meta-analysis: diagnostic performance of the combination of pepsinogen, gastrin-17 and anti-Helicobacter pylori antibodies serum assays for the diagnosis of atrophic gastritis. Aliment Pharmacol Ther. 2017;46(7):657-67.
  5. Alper A, Hardee S, Rojas-Velasquez D, Escalera S, Morotti RA, Pashankar DS. Prevalence and Clinical, Endoscopic, and Pathological Features of Duodenitis in Children. J Pediatr Gastroenterol Nutr. 2016;62(2):314-6.
  6. Tack J, Van den Houte K, Carbone F. Gastroduodenal motility disorders. Curr Opin Gastroenterol. 2018;34(6):428-35.
  7. Thompson CM, Young RR, Dinesdurage H, Suh M, Harris MA, Rohr AC, Proctor DM. Assessment of the mutagenic potential of hexavalent chromium in the duodenum of big blue rats. Toxicol Appl Pharmacol. 2017;330:48-52.
  8. Szymanska K, Gonkowski S. Bisphenol A-Induced changes in the enteric nervous system of the porcine duodenum. Neurotoxicology. 2018;66:78-86.
  9. Das KP, Wood CR, Lin MT, Starkov AA, Lau C, Wallace KB, et al. Perfluoroalkyl acids-induced liver steatosis: Effects on genes controlling lipid homeostasis. Toxicology. 2017 Mar 1;378:37-52.
  10. Shanh FG, Rahimnejad S, Bahrami A, Farhadian M. Risk Assessment of Workers’ Exposure to Volatile Organic Compounds in the Air of a Petrochemical Complex in Iran. Indian J Occup Environ Med. 2017;21(3):121-7.
  11. Wan X, Zhao Z, Qiu J, Guo Y, Wu J. Determination of epichlorohydrin in workplace air by gas chromatograph-electron capture detector. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2015;33(4):307-9.
  12. Mukhammadieva GF, Karimova LK, Bejgul NA, Bakirov AB, Valeeva ET, Mavrina LN. Osobennosti zagryazneniya vozdukha pri proizvodstve neprery`vnogo steklovolokna. Gigiena i sanitariya. 2016;95(6):548-51. [in Russian].
  13. Gavrilov VA, Luzin VI. Rost i formoobrazovanie nizhnej chelyusti u bely`kh kry`s razlichnogo vozrasta posle 60-dnevnogo vozdejstviya parov e`pikhlorgidrina. Mir medicziny` i biologii. 2014;10,4-2(47):93-7. [in Russian].
  14. Voloshina IS. Gistologichna budova vnutrishni`kh organiv reproduktivnoyi sistemi statevozrilikh shhuriv samcziv pislya trivalogo vplivu na organizm epikhlorgidrinu. Visnik problem biologiyi i mediczini. 2014;1(1):230-6. [in Ukrainian].
  15. Zhukov VI, Shherban NG, Nikolaeva OV. Vliyanie e`poksidsoderzhashhikh polioksipropilenpoliolov na geneticheskij apparat v usloviyakh dlitel`noj toksifikaczii v maly`kh dozakh. Mir medicziny` i biologii. 2015;11,4-2(54):123-7. [in Russian].
  16. Kuvenyova ML, Luzin VI, Morozov VN. Strukturnye izmeneniya slizistoj, myshechnoj obolochki i podslizistoj proslojki zheludka krys, voznikayushhie pod vozdejstviem epikhlorgidrina. Nauchnye vedomosti Belgorodskogo gosudarstvennogo universiteta. 2015;31,16(213):225- 9. [in Russian].
  17. Mirzebasov MA, Smirnov AS, Smirnov SN. State of duodenal mucosa in rats after epichlorohydrin long-acting. Journal of Education, Health and Sport. 2018;8(11):510-7.

Publication of the article:

«Bulletin of problems biology and medicine» Issue 4 Part 2 (154), 2019 year, 308-311 pages, index UDK 616.342 :546-3

DOI: