MODIFICATION OF THE FATTY ACID COMPOSITION OF PHOSPHOLIPIDS IN LIVER, MYOCARDIUM AND PLASMA TISSUES UNDER THE INFLUENCE OF IONIZING RADIATION AND WITH THE PRIOR APPLICATION OF A HYDROGEN SULFIDE DONOR
About the author:
Kovalchuk I. M., Gzhegotsky M. R., Rivis Y. F., Kovalchuk S. M.
CLINICAL AND EXPERIMENTAL MEDICINE
Type of article:
Fatty acid composition of phospholipids significantly affects on the functional state of cell membranes, transport function of cells, activity of multi enzyme systems that regulate intracellular metabolism. Polyunsaturated fatty acids (PUFA) maintain viscosity, structure and function of membranes, promote the synthesis of lipid mediators, coordinate metabolic processes, the expression of certain genes under normal conditions and the effects of stress factors, in particular ionizing radiation. It is known that the effect of low doses of radiation can lead to significant changes in the structure and dynamic activity of the fatty acid composition, interlipidic and protein-lipid interactions. A significant number of studies in recent years is associated with the study of mechanisms of regulatory effects of known cellular gas transmitters, in particular hydrogen sulfide (H2S). At the same time, in modern literature there is insufficient data on the development of adaptive reactions under conditions of exposure to ionizing radiation against the background of the use of this gas transmitter. Purpose and methods of research. The purpose of our study was to study the changes in the fatty acid composition of phospholipids in the tissues of the heart, liver and blood plasma under the influence of ionizing radiation and the pre-radiation exposure of the hydrogen sulfide donor. All experiments were carried out in compliance with the principles of bioethics in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals. The irradiation of animals in experimental groups was single-fractional, total, with total absorbed dose – 2 Gy. Determination of the fatty acid composition of phospholipids in the heart muscle, liver and blood plasma was performed by gas-liquid chromatography. Results and discussion. It has been established that the effect of a hydrogen sulfide donor (NaHS) in 30 minutes after its introduction leads to a decrease in the control group the level of saturated fatty acids phospholipids in blood plasma, liver tissue and, to a lesser extent, in the myocardium. In all investigated tissues, the level of short-chain saturated fatty acids: caprylic (C8:0), capric (C10:0), lauric (C12:0) was most reduced. However, an increase in the level of omega-3 and a decrease in the level of omega-6 PUFA was recorded. In particular, an increase in the content of omega-3 PUFA – eicosapentaenoicacid (C20:5) by 14% (in the liver), 12% (in the myocardium) and 19% (in blood plasma) in terms of control has been established. Significant reduction of omega-6 PUFA –eicosadienic acid (C20: 2) in all studied biological environments has also been established. With such changes in the composition of PUFA, the ratio of omega-3/omega-6 was significantly increased in liver tissue – by 10%, in myocardial tissue – by 11%, in the blood plasma – by 15%. One day after NaHS administration, the ratio of omega-3/omega-6 remains significantly higher than in control group in all of the studied biological environments, despite the tendency to decrease of previous lifetime of hydrogen sulfide donor. The effect of radiation after 24 hours leads to a significant increase in the level of short-chain saturated fatty acids of phospholipids than in control group, reduction of the content of omega-3 and increase in the content of omega-6 PUFA. It has been established that mentioned above changes of the level of omega-3 and omega-6 PUFA phospholipids under the influence of ionizing irradiation the ratio of omega-3/ omega-6 were significantly lower in comparison with controls in liver tissue – by 12%, in myocardium tissue – by 9%, in the blood plasma – by 11%. According to the pre-radiation exposure to NaHS, a tendency towards a decrease in the saturated lipid acids, an increase in the ratio of omega-3/omega-6 to the ionizing radiation effect was observed, but their values did not reach the level of control. Conclusions. The effect of introduction of hydrogen sulfide donor causes the modification of the fatty acid composition of phospholipids in liver, myocardial and blood plasma, which consists in increasing the content of omega-3 polyunsaturated fatty acids, increasing the ratio of omega-3/omega-6. Under the influence of ionizing radiation, the degree of saturation of fatty acids increases, and the ratio of omega-3/omega-6 decreases significantly, which causes the violation of micro-viscosity, fluidity and mobility of the lipid phase, which results in changes in membrane-dependent functional and metabolic processes. Prior to the radiation action, the introduction of the hydrogen sulfide donor (NaHS) causes a partial improvement of the fatty acid composition of the phospholipids in the liver, myocardium, blood plasma.
fatty acids, phospholipids, ionizing radiation, hydrogen sulfide donor, myocardium, liver, blood plazma
- Dlyaboha YuZ, Rivis YF. Zhyrnokyslotnyy sklad fosfolipidiv plazmy krovi, pechinky i skeletnykh m’yaziv shchuriv za eksperimental’noyi hiperkholesterynemiyi ta vplyvu ryb’yachoho zhyru. Biolohichni studiyi. 2011;2:73-84. [in Ukrainian].
- Gopanenko OO, Rivis YF. Zhyrnokyslotnyy sklad fosfolipidiv plazmy krovi i tkanyn za gostrogo argininovogo pankreatytu ta yoho korektsiyi. Eksperimental’na ta klinichna fiziolohiya ta biokhimiya. 2013;2:22-7. [in Ukrainian].
- Hryshchenko VA, Tomchuk VA. Fosfolipidnyy sklad vnutrishn’oyi membrany mitokhondriy enterjcytiv tonkoyi kyshky ta hepatocytiv za diyi na organism ionizuyuchoyi radiacii ta pry zastosuvanni liposom. Biologiya tvaryn. 2011;13(1-2):86-90. [in Ukrainian].
- Kalachnyuk L, Melnychuk D, Kalachnyuk H. Molekul’arni mekhanizmy rehul’uvannya syntezu. Metabolizmu y sekreciyi lipoproteyiniv u klitynakh pechinky. Visnyk L’vivskoho universytety. 2004;38:3-20. [in Ukrainian].
- Siasos G, Tousoulis D, Oikonomou E, Zaromitidou M, Verveniotis A, Plastiras A, et al. Effects of omega-3 fatty acids on endothelial function, arterial wall properties, inflammatory and fibrinolytic status in smokers: a cross over study. International Journal of Cardiology. 2013;2:340-6.
- Artamonov MV, Zhukov OD, Margitich VM, Klimashevsky VM, Hula NM. Vplyv ekzogennogo N-steroiletanolaminu na zhyrnokyslotnyy sklad individual’nykh fosfolipidiv izol’ovanogo sertsya shchuriv za umov postishemichnoyi reperfuzii. Ukr. biokh. zhurn. 2002;74(2):86-94. [in Ukrainian].
- Rivis YF. Obmin zhyrnykh kyslot u pechinci koropiv za riznoho rivnya zynku ta midi u kombikormi. Naukovyy visnyk LNUVMBH im. Gzhyts’koho. 2014;3(60):264-73. [in Ukrainian].
- Nazarov PE, Myagkova GI, Groza NV. Polinenasyshchennyye zhyrnyye kisloty kak universal’nyye endogennyye bioregulatory. Vestnik MITCHT. 2009;4(5):3-19. [in Russian].
- Shysh AM, Kukoba TV, Kharchenko OV. Modyfikaciya zhyrnokyslotnoho skladu membrane fosfolipidiv klityn omeha-3-polinenasychenykh zhyrnykh kyslot. Dop.NANU. 2004;11:184-8. [in Ukrainian].
- Schwenk RW, Holloway GP, Joost J, et al. Fatty acid transport across the cell membrane: Regulation by fatty acid transporters. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA). 2010. 82(4-6):1214-22.
- Osipenko AN, Akulich NV, Klishevich Fn. Zhyrnyye kysloty krovi i ich vzaimosvyazi pri ateroskleroze. Tavricheskiy medico-biologicheskiy vestnik. 2012;15(3):197-9. [in Russian].
- Hula NM, Marhitych VM. Zhyrni kysloty ta yikh pokhidni pry patolohichnykh stanakh. Kyyiv; 2009. 336 s. [in Ukrainian].
- Feng RT, Weng KL. Molecular mechanisms of low dose ionizing radiation in order to control bionegative effects to the organism and related human diseases. International journal of radiation biology. 2015;91:13-27.
- Ifigeneia VM, Danae AL, Frey B, Candéias SM, Gaipl US, Lumniczky K, еt al. Key mechanisms involved in ionizing radiation-induced systemic effects. A current review. Toxicology Research. 2016;5(1):12-33.
- Khyzhnyak SV, Hryshchenko VA, Stepanova LI. Aktyvnist’ fermentiv antyoksydantnoho zakhystu za diyi ionizuyuchoho vyprominenna ta fosfolipidvmisnoho preparatu. Fizyka zhyvoho. 2008;16(2):65-9. [in Ukrainian].
- Wallace JL, Muscara MN. Hydrogen sulfide: an endogenous mediator of resolution of inflammation and injury. Antioxid. Redox Signal. 2012;17:58-67.
- Kimura H. Hydrogen polysulfide signaling along with hydrogen sulfide (H2S) and nitric oxide (NO). J Neural Transm. 2016;123(11):1235-45.
- Magierovski M, Magierowska K, Hubalewska-Mazgaj M, Adamski J, Bakalarz D, Sliwowski Z, et al. Interaction between endogenous carbon monoxide and hydrogen sulfide in the mechanism of gastroprotection against acute aspirin-induced gastric damage. Pharmacol. Res. 2016;114:235-50.
- Shao M, Zhuo C, Jiang R, Chen G, Shan J, Ping J, еt al. Protective effect of hydrogen sulphide against myocardial hypertrophy in mice. Oncotarget. 2017 Apr 4;8(14):22344-52.
- Rivis YF, Fedoruk RS. Kilkisni khromatohrafichni metody vyznachennya okremykh lipidiv i zhyrnykh kyslot u biolohichnomu materiali. L’viv; 2010. 109 s. [in Ukrainian].
Publication of the article:
«Bulletin of problems biology and medicine» Issue 1 Part 2 (143), 2018 year, 130-137 pages, index UDK 612.123+611.127+611.36):(612.014.48+547.569)] – 019