INFLUENCE OF CADMIUM AND COPRUM ON MORTALITY INDICES IN RAT EMBRYOGENESIS AT INTRAGUNCARAL INTRODUCTION

Rudenko K. M., Kosse V. A., Trushenko A., Lyulko I. V., Topka E. G., Filippov Yu. A., Alekseenko Z. K.

BIOLOGY

Scentific article

In the modern world, the body of the inhabitants of urban areas is simultaneously affected by many harmful chemical compounds that are in the air, soil, water, food and are teratogenic, ie harmful to human and animal fetuses. Teratogenic factors act during certain critical periods of ontogenesis, especially during embryogenesis. Cadmium-containing substances are one of the most toxic heavy metal compounds that pollute the environment, feed and food and occur in dangerous concentrations in the atmosphere, lithosphere and hydrosphere due to manmade pressure on the environment. Under conditions of constant action of various exogenous factors, in particular heavy metals, the body needs to find effective protective agents. Thus, the urgent task of modern morphological and medical research is to determine the effect of heavy metal compounds on organ systems and the course of embryogenesis and to search for possible antagonists of their action. The aim of the study was to determine the effect of low doses of cadmium (2.0 mg/kg) and copper (0.1 mg/kg) when administered in combination throughout the pregnancy in rats. For research, females with a dated gestation period were obtained, and for this purpose, the estrous cycle of females was examined by the method of vaginal swabs. The solutions were administered by gavage, intragastrically, daily (once a day) from the first day of pregnancy: the first group – control, the second group – the introduction of a solution of cadmium chloride at a dose of 2.0 mg/kg – the group of isolated cadmium, and the third – the group of combined administration cadmium chloride, at a dose of 2.0 mg/kg, together with copper succinate at a dose of 0.1 mg/kg. The solution of copper succinate was nanoaquachelate, and the solution of cadmium chloride – ionic. Intrauterine survival was highest in the control group on the 19th day of embryogenesis (95.29%) and lowest in the group exposed to cadmium chloride at a dose of 2.0 mg / kg on the 19th day (75.07%). Analysis of the obtained data indicates a pronounced embryotoxic effect of cadmium chloride on the processes of embryogenesis, which is a significant increase in overall embryonic mortality, preimplantation and postimplantation mortality compared with the control group at all studied terms of embryogenesis.

cadmium, copper, embryotoxicity, embryos.

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«Bulletin of problems biology and medicine» Issue 3 (161), 2021 year, 101-105 pages, index UDK 612.3:591.39:661.852:661.782-092.9

10.29254/2077-4214-2021-3-161-101-105