ТHE ROLE CD68+ RECEPTOR ON CHANGES IN THE ACTIVITY OF MARKER ENZYMES MACROPHAGE POLARIZATION IN RAT TESTES AFTER LONG CENTRAL DEPRIVATION OF LUTEINIZING HORMONE

Stetsuk E. V., Shepitko V. I., Solovyova N. V., Akimov O. E.

MORPHOLOGY

Scentific article

Abstract. Introduction. The CD68+ cell receptor is part of the macrophage receptor family, which is associated with macrophages that are polarized by the M1 phenotype. The growth of the cell fraction of macrophages with CD68+ may not always explain all changes in the activity of marker enzymes, as cells of non-macrophage origin can also activate the transcription of iNOS and Arg genes under certain conditions. The aim of this study was to establish correlations between the number of CD68+ cells in the interstitial space and testicular vessels and the activity of iNOS and Arg, Sar production under conditions of central blockade of luteinizing hormone synthesis by triptorelin. Materials and methods. The study was performed on 40 adult male rats. Animals were randomly divided into 2 groups: control (10 animals) and experimental (30 animals). Animals in the experimental group were injected with a solution of triptorelin acetate at the rate of 0.3 mg of active substance per kg of animal weight to simulate the central derivation of luteinizing hormone synthesis. Animals from the experimental group were removed from the experiment on days 30, 90 and 180 of the simulation of the central derivation of luteinizing hormone synthesis with immunohistochemical examination of testicular macrophages for the presence of CD68 receptors. Results and discussion. 1. The relationship between iNOS activity and the prevalence of the CD68+ receptor in the testicular interstitium is strongly directly proportional. 2. The association between iNOS activity and CD68+ receptor prevalence in testicular vesselsisstrongly inversely proportional. 3. Sar production isinversely proportionalto the prevalence of the CD68+ receptor in the testes. 4. Sar production is directly correlated with the prevalence of the CD68+ receptor in the testis interstitium. Conclusions. Macrophages bearing the CD68 receptor on their surface are a source of nitricoxide in the testes of rats on days 30 and 90 of central deprivation of luteinizing hormone synthesis. CD68+ cells are active producers of superoxide anionradical on days 90 and 180 of central deprivation of luteinizing hormone

triptorelin, CD68+ , nitricoxide, superoxide anionradical, arginase, luteinizing hormone.

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«Bulletin of problems biology and medicine» Issue 3 (161), 2021 year, 277-281 pages, index UDK 612.0616.21:903.349.8

10.29254/2077-4214-2021-3-161-277-281