THE EFFECT OF ZINC SULFATE, SODIUM THIOSULFATE, LIPOIC ACID, TAURINE ON THE EXPRESSION OF ENZYMES OF HYDROGEN SULFIDE SYNTHESIS, MEDIATORS OF INFLAMMATION, FIBROGENESIS IN THE KIDNEYS OF RATS WITH DIET-INDUCED OBESITY

Blazhchenko V.V., Zaichko N.V.

HYGIENE AND ECOLOGY

Scentific article

Introduction. The role of hydrogen sulfide (H2 S) in the pathochemistry of obesity and its complications remains the subject of active research in biology and medicine. The kidneys are one of the key target organs that suffer from obesity. Physiologically, H2 S is synthesized in the kidneys, and is involved in the regulation of all renal functions, lipid metabolism and adipogenesis. The search for correctors of H2 S metabolism, which would provide nephroprotection in obesity, would not activate lipogenesis and adipogenesis, is relevant.The aim of study: to establish the effect of zinc sulfate, sodium thiosulfate, lipoic acid, taurine on the expression of enzymes of hydrogen sulfide synthesis, mediators of inflammation, fibrogenesis in the kidneys of rats with diet-induced obesity. Object and methods of research. The experiments were performed on 60 white male laboratory rats in accordance with the principles of bioethics (Strasbourg, 1986). Obesity was simulated by a high-calorie high-fat diet (4.33 kcal/g, 39.5 % fat) for 70 days. Control rats received standard feed (2.71 kcal/g, 10.8 % fat). Metabolic correctors (zinc sulfate, sodium thiosulfate, lipoic acid, taurine) were administered intravenously from the 56th to the 70th day. The level of cystatin C in the blood was determined, the expression of H2 S‑synthesizing enzyme genes (CSE, CBS), IGF1 and TNFα levels were determined in the kidneys. Statistical processing was performed in the package IBM Statistics SPSS 26. Results. In obese rats, increased serum cystatin C levels, decreased levels of CSE and CBS gene expression, increased levels of inflammatory and fibrogenesis mediators (IGF1, TNFα) were found. Inverse associations between the level of gene expression of H2 S‑synthesizing enzymes in the kidneys and biochemical markers of kidney damage have been identified. Zinc sulfate (124 mg/kg), sodium thiosulfate (300 mg/kg), lipoic acid (100 mg/kg), and taurine (100 mg/kg) decreased cystatin C, IGF1, and TNFα levels in obese rats. Zinc sulfate and lipoic acid increased the expression of CSE, CBS genes in the kidneys of obese rats, thiosulfate and taurine did not cause this effect. The introduction of any of the correctors did not aggravate the signs of obesity. Conclusions: Decreased expression of H2 S synthesis enzymes is associated with activation of inflammatory and fibrotic processes in the kidneys under obesity. Zinc sulfate, lipoic acid, thiosulfate, taurine reduce biochemical changes in the kidneys, but show different activity in influencing the expression of H2 S metabolic enzymes.

hydrogen sulfide, obesity, kidney, inflammation, fibrosis.

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«Bulletin of problems biology and medicine» Issue 2 Part 1 (164), 2022 year, 114-125 pages, index UDK 546.221.1:616.61–056.52:612.08

10.29254/2077-4214-2022-2-1-164-114-125