CHANGES IN THE STRUCTURE OF PERIODONTAL COMPONENTS IN THE MODELING OF ACUTE PERIODONTITIS IN RATS AND ITS CORRECTION BY STEM CELLS

Biguliak Н. Т., Klishch I. M.

MORPHOLOGY

Scentific article

The aim of our research was to study peculiar features of morphological changes in the components of the periodontium in the modelled acute periodontitis and its correction by stem cells. The results of histological study of periodontal tissues of the animals with acute experimental periodontitis, which was corrected using human mesenchymal stem cells were analysed. Depending on the treatment, the animals were divided into 4 groups: the 1st group – the animals without pathology (intact); the 2nd – the animals with modelled acute periodontitis; the 3rd – the animals with periodontitis corrected by human mesenchymal stem cells. The material for histological study was obtained on the 1st, 7th, 14th and 21st days after taking the animals out of the experiment. Periodontal tissues of the animals were used as the material for morphological studies. For photo documentation, the images of histological specimens were displayed on a computer monitor using a Nicon Eclipse C microscope and a SCMOS Digital Camera by means of ToupWiev. In the experimental modelling of acute periodontitis the destructive changes in the epithelium and connective tissue stroma, impaired blood flow, inflammatory response in the gum tissue were present. According to the results of the research, it was established that correction by human mesenchymal stem cells positive effect on a course of the modeled pathological process. The morphological changes characteristic of periodontitis gradually reduced, i.e. the normal blood supply to the tissues restored, the arteries were less full-blooded, the tone of their walls decreased. Consequently, the swelling of the tissues gradually decreased becoming of original size. The polymorphonuclear infiltration fields decreased and gradually disappeared. Osteoblasts promoted restoration of the bone structure of the jaw alveolar process. The size of the periodontal pocket decreased, and in some areas it fully closed as a result of complete adhesion of the mucous membrane of the gums to the tooth, which rose on its surface upwards.

acute experimental periodontitis, stem cells, regeneration.

1. Hodovana OI. Zakhvoryuvannya parodontu (hinhivit, parodontyt, parodontoz). Dzhura; 2009. 200 s. [in Russian].
2. Zabolotnyy TD, Borysenko AV, Pupin TI. Zapalʹni zakhvoryuvannya parodontaabolotnyy. HalDent; 2013. 205 s. [in Ukrainian].
3. Guzman YA, Sakellari D, Arsenakis M, Floudas CA. Proteomics for the discovery of biomarkers and diagnosis of periodontitis: a critical review. Expert review of proteomics. 2014 Feb 1;11(1):31-41.
4. Thumbigere'Math V, Michalowicz BS, Hodges JS, Tsai ML, Swenson KK, Rockwell L, et al. Periodontal disease as a risk factor for bisphosphonate'related osteonecrosis of the jaw. Journal of periodontology. 2014 Feb;85(2):226-33.
5. Hrudyanov AY, Sysoeva VYu, Ternovoy YuV. Stvolovye kletky y vozmozhnosty ykh prymenenyya v parodontolohyy. Stomatolohyya. 2012;91(1):71-5. [in Russian].
6. Zolotukhyna EL. Stvolovye kletky y perspektyvy ykh prymenenyya v stomatolohyy y chelyustnolytsevoy khyrurhyy. Molodoy uchenyy. 2014;9(6):145-7. [in Russian].
7. Butenko HM, Kyryk VM. Reheneratyvna medytsyna i stovburovi klityny – problemy i rishennya. Zhurn. NAMN Ukrayiny. 2011;17(1):62-6. [in Ukrainian].
8. Honda MJ, Imaizumi M, Tsuchiya S, Morsczeck C. Dental follicle stem cells and tissue engineering. Journal of oral science. 2010;52(4):541- 52.
9. Hammarström L. Enamel matrix, cementum development and regeneration. Journal of clinical periodontology. 1997 Sep;24(9):658-68.
10. Guida L, Annunziata M, Carinci F, Di Feo A, Passaro I, Oliva A. In vitro biologic response of human bone marrow stromal cells to enamel matrix derivative. Journal of periodontology. 2007 Nov;78(11):2190-6.
11. Renard E, Lopez-Cazaux S, Guicheux J, Weiss P, Laboux O, Alliot-Licht B. Stem cells of dental pulp. Comptes rendus biologies. 2007 Aug 13;330(9):635-43.
12. Kozhemʼyakin YuM, Khromov OS, Filonenko MA, Sayfetdinova HA. Naukovo-praktychni rekomendatsiyi z utrymannya laboratornykh tvaryn ta roboty z nymy. K.: Avitsena; 2002. 156 s. [in Ukrainian].
13. Bahriy MM, Dibrova VA, Popadynetsʹ OH, Hryshchuk IM. Metodyky morfolohichnykh doslidzhenʹ: monohrafiya. Vinnytsya: Nova knyha; 2016. 238 s. [in Ukrainian].

«Bulletin of problems biology and medicine» Issue 2 Part 2 (165), 2022 year, 22-31 pages, index UDK 616.314.17-002.1-091.8-085.361-092.9

10.29254/2077-4214-2022-2-2-165-22-31