IMMUNOGISTOCHEMICAL CHARACTERISTICS OF AMNION IN CASE OF COMPLICATIONS OF PREGNANCY AND ANTEINTRANATAL FETAL DEATH

Zats O. S.

MORPHOLOGY

Scentific article

The outcome of pregnancy and childbirth largely depends on the integrity of the fetal membranes that ensure the fetal existence. During pregnancy on the background of preeclampsia, iron deficiency anemia in pregnant women, acute chorioamnionitis increases the risk of premature rupture of membranes, which is associated with 40- 75% of perinatal losses. The aim of the study was to determine the immunohistochemical and morphometric features of the epithelium and mesoderm of the amnion at stillbirth in pregnant women with preeclampsia, iron deficiency anemia and acute chorioamnionitis. Object and methods of research. 58 membranes of ante-intranatally dead fetuses were studied, which were divided into 6 clinical groups: membranes of premature (n = 8) and full-term (n = 8) fetuses of pregnant women with preeclampsia, premature (n = 8) and full-term (n = 8) fetuses pregnant women with iron deficiency anemia, premature (n = 10) and full-term (n = 16) fetuses with acute chorioamnionitis. The control group consisted of 8 shells during physiological pregnancy with the birth of a live full-term baby. Immunohistochemical evaluation of the cellular composition of the amnion was detected by the expression of the marker of epithelial cells of cytokeratin (Cytokeratin PAN AE1/AE3), the marker of mesenchymal cells of vimentin (Vimentin V9), using MCA to collagen types I, III, IV detected interstitial. Levels of expression of cytokeratin in amniotic epithelium, collagen I, III types in amniotic mesoderm were determined. The micropreparations were examined in an Olympus BX-41 microscope (Japan). Statistical processing was performed using the software package Statistica 6.0 and Microsoft Excel 2003. To compare the parameters used non-parametric methods (U-test Mann-Whitney). With the obtained results, a correlation analysis was performed between the estimated values to determine the direction and strength of the connection. Results. Analysis of immunohistochemical reactions revealed in fetal membranes in physiological pregnancy and pregnancy complicated by preeclampsia, chorioamnionitis and iron deficiency anemia in pregnant women, signs of epithelial-mesenchymal transformation of amniotic epithelium, which were characterized by increased . In the control group with physiological pregnancy and in clinical groups with full-term pregnancy, a strong positivecorrelation was found between the level of cytokeratin expression by the amniotic epithelium and the expression levels of interstitial collagen types I and III in the amniotic mesoderm (p <0.5 for all groups). due to the acquisition of the amniotic epithelium properties of mesechymal cells with collagenolytic activity. Conclusions. As a result of the study, data were obtained on the epithelial-mesenchymal transformation of the amniotic epithelium with the acquisition of collagenolytic properties, which are exacerbated by complications of pregnancy with preeclampsia, iron deficiency anemia and acute chorioamnionitis. The obtained data may be useful for further study of the mechanism of premature rupture of amniotic membranes in complicated pregnancy and prevention of related complications from the mother and fetus.

preeclampsia, anemia, chorioamnionitis, fetal membranes, immunohistochemistry

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«Bulletin of problems biology and medicine» Issue 4 (162), 2021 year, 266-271 pages, index UDK 616.3:616.12-008.331.1:616-005.98:618.333-091.8

10.29254/2077-4214-2021-4-162-266-271