THE FEATURES OF EPITHELIAL-MESENCHYMAL TRANSFORMATION IN COLORECTAL CARCINOMAS AS PROGNOSTIC CRITERIA OF RECURRENCE AND LETHAL OUTCOME

Yakovtsova I. I., Yakymenko A. S., Ivakhno I. V., Danyliuk S. V.

PATHOMORPHOLOGY

Scentific article

Epithelial-mesenchymal transformation (EMT) is a prognostic criteria of colorectal carcinoma (CRC). However, its accounting has not been widely used in clinical practice. There is also no information about stages of EMT as a prognostic criteria of CRC. The aim of the research was to investigate the prognostic criteria of recurrence and lethal outcome of CRR IIA and IIIB stages. Material and Methods. The group I was formed from primary CRC without recurrences. The ІІ group – primary CRC with recurrences; ІІА – with recurrences during 5 years from the moment when the tumor was removed, without fatal outcome; ІІВ – with recurrences and fatal outcome from generalization of tumor process during 5 years from lethal outcome from the moment when the tumor was removed. The ratio of tumors with certain differentiation in groups of research was taken equal. Immunohistochemical study was performed using monoclonal antibodies to vimentin, CC20, E- and N-cadherin. Taking into account the coexpression of markers allowed to determine the stages of EMT. Results. Molecular biological signs of EMT were observed in 53.3% (32/60) of CRC stage IIA-IIIB, its appearance was associated with G3 differentiation (p<0.03). Also, CRC G3 was characterized by a higher stage of EMT in comparing with CRC G1-2 (p<0,05). The appearance of EMT was associated with recurrence of CRC (p<0.01), there is a tendency of increasing the stage of EMT among recurrent CRC, mainly due to a subgroup of recurrent with lethal outcome. The appearance of EMT was a prognostic criterion of recurrence free survival (RFS). According to the data received, the rate of RFS in patients with EMT of CRC was lower (p<0.001). Thus, the average recurrence period among those without EMT was 25.7±3.9, among CRC with EMT – 17.4±2.7 months. An inverse correlation was also observed between the recurrence period and the EMT stage (r=-0.42, p<0.01). There is a tendency to a greater number of cases of CRC stage 3-4 EMT among patients with recurrence and death (p>0.05). Conclusions. EMT is a prognostic criterion for recurrence and recurrence time of CRP pT3N0-2M0, more common in tumors of patients with recurrence and death.

EMT, colorectal cancer, recurrence, prognosis.

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«Bulletin of problems biology and medicine» Issue 4 (162), 2021 year, 297-301 pages, index UDK 616.34/.35-006.6-076-091.8

10.29254/2077-4214-2021-4-162-297-301