ACTIVITY OF MARKER ENZYMES OF MACROPHAGES’ POLARISATION IN ORAL FLUID OF CHILDREN WITH TYPE 1 DIABETES MELLITUS IN THE DYNAMICS OF TREATMENT OF CHRONIC CATARRHAL GINGIVITIS

Kuz I. O., Sheshukova O. V., Akimov O. Ye., Kostenko V. O., Maksymenko A. I., Pysarenko O. A.

DENTISTRY

Scentific article

The local changes in periodontal tissues in persons with diabetes mellitus are characterized by increased production of reactive oxygen species and proinflammatory cytokines. The accumulation of glycation products and their active interaction with receptors lies in above-mentioned processes. One of the central roles in the development of inflammation is played by macrophages, acquiring, depending on the microenvironment, and pro-inflammatory M1 or anti-inflammatory M2 phenotype. The aim of the study was to determine the NO-synthase and arginase activity in oral fluid in children with type 1 diabetes mellitus under the influence of the treatment scheme for chronic catarrhal gingivitis. Materials and methods. The study involved 56 children with type 1 diabetes mellitus and 26 non-diabetic children. The activity of NO synthase (NOS), arginase activity, PMA index in the Parma modification (1960) was determined. Results. The using of our treatment scheme in children with chronic catarrhal gingivitis and type 1 diabetes mellitus lead to a change in the polarization of oral macrophages towards the predominance of M2 polarization after 1 month. It is evidenced by increased arginase activity and a statistically significant decrease in iNOS. The polarization of macrophages changed to the predominance of M1 polarization activity after 1 year. It is also evidenced by the decrease in arginase activity, and absence of changes in the activity of iNOS. Conclusions. We have developed a scheme for the treatment of chronic catarrhal gingivitis in children with type 1 diabetes mellitus. It normalizes the polarization of oral macrophages caused by exposure to chronic catarrhal gingivitis as a local pathogenetic factor.

type 1 diabetes mellitus, gingivitis, macrophages, NO-synthase, arginase.

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«Bulletin of problems biology and medicine» Issue 4 (162), 2021 year, 315-319 pages, index UDK 616.379-008.64:616.311.2-053.2

10.29254/2077-4214-2021-4-162-315-319