IMMUNOHISTOCHEMICAL STUDY OF MACROPHAGE LINK OF LOCAL PROTECTION OF SPHENOIDAL SINUS MUCOSA IN ELDERLY PEOPLE USING CD-68 MARKER
About the author:
Pronina O. M., S. N. Sovhyrya, Pyrog-Zakaznikova A. V., Polovik A. Yu., Vinnik N. і .
Heading:
CONTENTS
Type of article:
Scentific article
Annotation:
The purpose of the research was to study the features of localization of infraepithelial macrophages in various areas of human sphenoidal sinus mucosa. The object and methods of research. The object of research was the sphenoidal sinus mucosa of people, died of circumstances, not connected with ENT-pathology. Homogeneous antibodies have been used as primary ones to the CD-68 markers of macrophages, histiocytes (КР1-clone, Dako). As was known, immunological status in the epithelial layer of pseudostratified ciliated cylindrical epithelium is provided by the cells, representatives of immunological monitoring, called infraepithelial macrophages. Judging by the theoretical statement, localization of these cells, mostly ciliated and goblet ones, in the areas of sphenoidal sinus mucosa and growth zones has been studied due to CD-68 immunohistochemical marker. Results of the research and their discussions. The results of immunohistochemical studies of various areas of human sphenoidal sinus mucosa, using the CD-68 marker, founded the mechanism of localization of infraepithelial macrophages in the pseudostratified ciliated cylindrical epithelium: most of the macrophages are located in the areas, covered by the ciliated cells. Areas, covered by the goblet cells and in the growth zones, have less macrophage cells that, in our opinion, is explained by the immunological monitoring due to integration of various types of lymphocytes. Conclusions. It is planning to conduct quantitative evaluation of tissue macrophages of mucosa of various walls of human sphenoidal sinus and study the features of all chains of immunological monitoring in the pseudostratified ciliated cylindrical epithelium of human sphenoidal sinus.
Tags:
macrophage, pseudostratified ciliated cylindrical epithelium, CD – 68 marker
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Publication of the article:
«Bulletin of problems biology and medicine» Issue 2 part 2 (108), 2014 year, 102-104 pages, index UDK [611. 21+611. 018]:616-053. 9