Mykytyuk O. P.

Daily and Seasonal Profiles of Some Connective Tissue Metabolism Values in Patients with Osteoarthritis

About the author:

Mykytyuk O. P.



Type of article:

Scentific article


Diagnostic tests in planned examinations and routine calibration procedures are taken at morning hours usually; but those obtained during the day, including urgent cases have to be compared with standard without regard on possible daily variations. Osteoarthritis is a chronic wide-spread disease of joints where circadian variations of symptoms intensity as well as diurnal variations in target organs growth and metabolism were reported. Detection of daily and seasonal variations of some metabolites of connective tissue routinely employed in test-ing was the aim of our investigation. 88 patients suffering from osteoarthritis were examined. Daily and seasonal changes of collagenolytic activity of blood serum, blood content of syalic acids and hexosamines in healthy persons and patients with osteoarthritis were investigated. Tests were performed with 6 hour interval within 24-h on first day in clinic, results were compared with those from 32 volunteers who had no acute illnesses or signs and anamnesis of chronic pathology within 3 months interval before investigation. All results were approved statistically. Marked differences comparing profiles of healthy volunteers were shown (abolishment of circadian rhythm; night increase up to 1. 5 times and daily increase over 30%). Content of syalic acids increased by 57,4% (at 2.00), and day time values – by 35% (at 14. 00). Hexosamines content increased by 41,3 – 63,2% at night hours and by 39,0- 56,5% at day time in different seasons. Changes of collagenolytic activity and increase of blood level of connective tissue metabolites co-incided at same time period in case of osteoarthritis. All rhythms of patients with osteoarthritis became inverse comparing control profiles. Presence of synovyitis caused deletion of daily fluctuations of all investigated parameters and increased their absolute values. Maximal changes of all investigated indices were observed in autumnal period – they were statisti-cally approved and significant throughout 24h cycle. Possible mechanisms for circadian periodicity of cartilage metabolism in health and arthritis are discussed as well as changes leading to rhythms disturbances in case of pathology. Conclusions were done that evening and night hours are time when maximal changes of biochemical values are observed in case of osteoarthritis (time interval 22. 00-2. 00). Increased content of metabolites at night hours is marked and significant though the whole year, and increased through day time in autumn only. Established variations should be taken into account both in clinical practice and research in order to avoid misinterpretation.


osteoarthritis, daily rhythms, seasonal rhythms.


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Publication of the article:

«Bulletin of problems biology and medicine» Issue 2 part 3 (109), 2014 year, 170-174 pages, index UDK 616. 72-007. 24:612. 751. 3. 015. 3