The Influence of Thiotriazoline on the Cytokine Status Disturbances in the Blood Serum under the Con- dition of Experimental Bronchial Asthma

Regeda M. S., Kolishetska M. A., Kovalyshyn O. A.

CONTENTS

Scentific article

Bronchial asthma (BA) is one of the most actual problems of modern paediatrics, therapy and allergology The general representation of its pathogenesis is the chronic eosinophilic inflammation of the respiratory tract theory. The dominant role in its development is played by pro-inflammatory cytokines, which are glycosylated polypeptides. They regulate and determine the nature of the immune response. The acute inflammatory response is initiated as a result of the tissue macrophages activation and secretion of inflammatory cytokines, including tumor necrosis factor – α (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6), which are the reasons of many local and systemic changes obtained during the acute inflammatory response activation. The course and intensity of the inflammatory response is controlled by several cytokines. IL-1 subtype provides an appropriate protective response in case of the pathogenic factors acting. The features of providing an inflammatory infection with cytokine are very actual subject of scientific research. According to modern ideas, there are some significant changes of maintaining homeostasis mechanisms – a system of lipid peroxidation – antioxidant protection and balance of cytokines during the bronchopulmonary diseases in the respiratory tract. Nowadays, experimentalists and clinicians have attracted the attention with their researches. They are concerning to the influence of medicine that has immune and antioxidant properties. So the research of thiotriazoline influence on the treatment of experimental asthma and on the indicators and cytokine status should be provided. The aim of our research was to determine the character of the disturbances content level of pro-inflammatory interleukin-6, tumor necrosis factor-α and anti-inflammatory cytokine interleukin-10 (IL-10) in the serum of guinea pigs and estimation of thiotriazoline influence on the cytokine profile in the conditions of formation of experimental bronchial asthma. Materials and methods. Researches were conducted on guinea pigs, divided into six groups: I – control, II – 5th day of experiment, III – 19th day of bronchial asthma development, IV – 26th day and V – 33 d day of model process, VI- guinea-pigs with BA on 33 days after treatment with thiotriazoline which was administered intramuscularly at a dose of 100 mg weight for 10 days. Experimental model of bronchial asthma was restored on guinea-pigs by the V. I. Babych method (1979). Euthanasia of animals was carried out by the way of decapitation followed the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes (1985). The IL-6, IL-10 and TNF-α concentration in the blood serum was defined for all groups of guinea-pigs. It was made using hard-phase immune-enzyme analysis (ELISA), by means of the test-system “Diaclone” (France). Numerical results were adapted with static method using Stjudent’s criteria. Results and discussion. At the study of the system of cytokines it was set at experimental BA, that in all probed days of experiment the reliable changes of concentration of both pro-inflammatory and anti-inflammatory cytokines took place in comparing to the group of intact animals. The increasing of pro-inflammatory activity of cytokines is set, at the same time, decreasing of anti-inflammatory IL-10. The introduction of thiotriazoline result in the decreas- ing of IL-6 on 43, 7% (р0,05), TNF-α on 41,9% (р0,05) and substantial increasing of IL-10 on 86,1% (р0,05) in as compared to the group of animals which was not added influence of this preparation. Conclusions. It has been determined significant advantage of pro-inflammatory cytokine activity, which increas- ing according the development of the pathological process and, simultaneously, reducing the anti-inflammatory IL-10. It has been detected corrective action of thiotriazoline on the investigated indices of cytokine profile.

bronchial asthma, cytokines, thiotriazoline

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