Dependence between Indicators of Pro- and Antioxidant System in Rat Kidneys Associated with Sulema Nephropathy in Case of Salt Loading

Velyka A. Ya.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Activation of lipid peroxidation causes significant changes in cell metabolism and in functions of bio- membranes and is an important element in pathogenesis of many diseases, including those of kidneys. We have shown that in conditions of 3 % salt loading in rats’ kidneys processes of free radical oxidation undergo changes. The state of free radical oxidation (FRO) of lipids and some ferments of antioxidant protection system in rats’ kid- neys in presence of sublimate in conditions of 3 % salt loading remains unclear. That’s why the goal of this research was to find probability of connection between indicators of free radical oxidation (FRO) of lipids and indicators of antioxidant protection system of rats’ kidneys in presence of sublimate during salt loading. In conditions of 3 % salt loading in rats’ kidneys it was found that the content of thiobarbituric acid in renal cortex grows on 70 % in comparison with the control sample. The enzymes of antioxidant protection system also had some changes: growth of glutathione S-transferase activity on 45 % was noted. Simultaneously, catalase in renal cortex in the same conditions lowered on 77 % in comparison with the control sample. In conditions of salt loading on the background of sublimate intoxication further growth of thiobarbituric acid was noted – its content doubled. After effects of sublimate, salt loading led to even greater changes of indicators of macromolecules free radical oxidation processes. Glutathione S-transferase activity stayed in high numbers and was higher than control samples on 55 %, but was not much different from indicators of salt loading only. Catalase activity on the background of sublimate nephropathy did not change in comparison with the control sample. Multivariate regression dependence between activity of catalase, content of thiobarbiturate-reaction products and activity of glutathione S-transferase in renal cortex in conditions of sublimate nephropathy and 3 % salt loading with solution of sodium chloride has been found. Therefore, antioxidant system of organism takes part in disposal of active oxygen forms. Intoxication of rats with solution of sublimate leads to destruction of cell membrane and causes activation of macromolecules’ free radi- cal oxidation process. In the renal cortex changes of thiobarbituric acid reaction products’ content and activity of antioxidant protection enzymes (glutathione S-transferase and catalase) were fund. These results are confirmed by multivariate regression analysis. It was found that glutathione S-transferase activity in rats’ renal cortex in condi- tions of 3 % salt loading on the background of sublimate intoxication grew in approximately 1.5 times in comparison with the control sample. Salt loading led to probable decrease of catalase activity in renal cortex in comparison with the control sample, while the effect of 0.1 % sublimate solution in dose of 5 mg per kg of body weight of the animal did not change the activity of this enzyme. Clear connection between thiobarbiturate-reaction products, activity of catalase and glutathione S-transferase in renal cortex in conditions of sublimate nephropathy with 3 % loading with solution of sodium chloride was found by multivariate regression analysis. Antioxidant system of organism takes part in disposal of active oxygen forms. Intoxication of rats with solution of sublimate leads to destruction of cell membrane and causes activation of macromolecules’ free radical oxidation process. In the renal cortex changes of thiobarbituric acid reaction products’ content and activity of antioxidant protection enzymes (glutathione S-transferase and catalase) were fund. These results are confirmed by multivari- ate regression analysis.

sublimate, catalase, glutathione S-transferase, thiobarbiturate-reaction products, regression analysis.

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