Stokes-Polarimetric Mapping of Orientation Construction of Histologic Sections of Human Fetuses’ Paraorbital Cellular Tissue

Sobko O. V., Olijnyk I. Yu., Ushenko A. G.

METHODS AND METHODOLOGIES

Scentific article

Objective. An analysis of the available scientific literature shows that at this stage there are not any studies of organs and structures in the orbital area of human prenatal ontogenesis by means of laser polarimetry of biological tissues, which makes this study relevant and timely. In consideration of the importance of developing the base of perinatal anatomy and of capabilities of modern research methods, of information technology in medicine we were the first to propose the method of Stokes-polarimetric statistical analysis of microscopic images of histological sections of human fetuses’ paraorbital cellular tissue when fetal growth reached 5-10 months. Material and methods of research. To describe the topographic structure of classical microscopic images of tissue sections of paraorbital cellular tissue of 5-10 month fetuses, we used polarized laser radiation with subsequent calculation of a set of mathematical parameters of Stokes vector, which is the most informative to describe the orientation and polycrystalline structure of biological preparations. To objectively characterize the coordinate distributions in the plane of biological preparation of Stokes vector parameters, the statistical points of the 1st – 4th order were calculated by a standard software MATLAB 6. Among the set of Stokes vector parameters we chose the coordinate distribution of values of the second Stokes vector parameter ( S2 ), which has the most complete information about the orientation construction of its material in the dots of the microscopic image. Results. The analysis of the experimental research results of the statistical structure of coordinate distributions of a set of orientation parameters S2 which characterize the degree of ordering in the substance of histological sections of paraorbital eyepit cellular tissue at different stages of fetal growth, showed a wide range of variation of random values of orientation parameter S2 within the plane of histological sections of paraorbital cellular tissue-1S21. Time history(from 5 months to 10 months) of such a structure is found in a more structured material of the eyepit paraorbital cellular tissue. This effect illustrates an increase in the likelihood of orientation parameter values S2 , that are different from zero – S2 0. Within the statistical approach, in terms of quantity, the processes of morphological evolution of paraorbital cellular tissue illustrate histograms of the random orientation parameter values S2 distribution. Extrema of histograms N S2 of histological sections samples of paraorbital cellular tissue are localized in the area S2 0 . Comparative analysis of the obtained data about the statistical structure of the distribution of the paraorbital eyepit cellular tissue orientation parameter S2 in different periods of fetal development showed diagnostic sensitivity of a set of the objective statistical moments of the 1st – 4th order, characterizing the degree of order in morphological construction material of the object. Most sensitive to changes in the degree of substance ordering in paraorbital eyepit cellular tissue of a fetus at different stages of growth were found statistical moments of the 2nd – 4th order which characterize the coordinate distributions of the orientation parameter of corresponding eyepit biological preparations microscopic images. Conclusions. It was for the first time that we proposed the method of Stokes-polarimetric statistical analysis of microscopic sections of the eyepit paraorbital cellular tissue in the fetuses at different stages of their growth and we used an objective statistical analysis (calculation of statistical moments of the 1st-4th order) of the Stokes vector parameters coordinate distributions. It was for the first time that orientation parameter S2 was suggested for the objective evaluation of the morphological construction of a set of biological preparations in the fetal eyepit at dif- ferent stages of its growth. Experimentally examined the coordinate distributions of the orientation parameter S2 in histological sections of the fetal eyepit paraorbital cellular tissue in different periods of its growth. The basic re- lationship between changes in a set of statistical moments of the 1st – 4th order which characterize the coordinate distributions of the orientation parameter and characteristics of temporal spatial structuring of agents of the fetal eyepit at different stages of its growth was established–as to the orbital cellular tissue, the statistic moments of the 2nd-4th order proved to be the most sensitive with the following range of changes in their values Z2 S2 2,23; Z S 3,08;  3 Z 3 S4 2,06.

fetus, paraorbital cellular tissue, eyepit, Stokes-polarimeter, orientation parameter, Stokes vector, statistical moment

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«Bulletin of problems biology and medicine» Issue 3 part 3 (112), 2014 year, 229-234 pages, index UDK 616-053. 15-018:[535. 361:535. 51]