COMPARATIVE CHARACTERISTICS OF ANTIMICROBIC ACTION OF HERBAL MEDICINES

Derevianko T. V., Ananiyeva M. M., Faustova M. O., Loban G. A.

MICROBIOLOGY

Scentific article

Recently there has been a clear upward trend in various herbal remedies that have several advantages over synthetic agents and are widely used in the complex treatment of various diseases. Therefore, the question of finding new substances that are highly effective for microorganisms and harmless to humans is urgent. An alternative to chemicals may be herbal preparations that contain biologically active substances and effectively affect pathogenic and conditionally pathogenic microorganisms, exhibit a wide range of therapeutic and prophylactic effects. Among herbal remedies we have attracted the attention of herbal extracts Sangviritrin and Sandrin, which are used in the local treatment of infectious and inflammatory diseases, in particular in dentistry, otolaryngology and dermatology. We have also investigated an under-studied herbal drug Neonox cyclamen. The results of the study of antibacterial and antifungal activity of herbal medicines against museum strains of microorganisms Staphylococcus aureus АТСС 25923, Escherichia coli АТСС 25922, Candida albicans ATCC10231 are presented in the article. Antimicrobial activity of the active substances of herbal medicine was determined by the method of diffusion into agar. In the process of determining the antimicrobial action of the studied herbal medicines (Sandrin, Neonox cyclamen, Sangviritrin), we noted their different activity against museum strains of gram-positive and gram-negative microorganisms. Among the studied herbal medicinal products, Sandrin has the most significant antimicrobial effect on museum cultures of gram-positive (S. aureus, C. albicans) and gram-negative (E. coli) microorganisms, which is due to halothanins, which have the ability to bind to microorganisms and lead to a decrease in activity and inhibition of cell wall efflux pumps. It is noted that Sangviritrin also showed significant antimicrobial activity against S. aureus and C. albicans, although it was inferior to the Sandrin effect. For E. coli strain growth inhibition under the action of Sangviritrin was not observed, which indicated its resistance to the active substances of the investigated herbal medicine. As the producers indicate, the mechanism of the antimicrobial action of Sangviritrin, which is more pronounced in relation to gram-positive microorganisms, is the damage of the cell wall structure, which leads to disturbance of the processes of its permeability and membranes of division.A negative result on the effect on all studied museum strains of microorganisms was demonstrated by Neonox with cyclamen extract, since there were no growth retardation zones of S. aureus, C. albicans, E. coli. We have not detected any antibacterial and antifungal activity of this herbal medicine. The action of cyclamen saponins and other excipients is more aimed at irritating the nerve endings, which leads to intense mucus formation and its removal from the nasal sinuses, and consequently a decrease in microbial contamination. This reduces the fluid content of the cells, reduces swelling and restores nasal breathing.

antibacterial action, antifungal action, herbal medicines, resistance, museum strains of microorganisms.

1. Vrynchanu NO. Rolʹ eflyuksnykh system u rezystentnosti mikroorhanizmiv do antybiotykiv. Farmakolohiya ta likarsʹka toksykolohiya. 2017;3(54):3-15. [in Ukrainian].
2. Davidenko K. Novi antybiotyky: mekhanizmy diyi ta rezystentnosti. Ukrayinsʹkyy medychnyy chasopys [Internet]. 2019;08-01. Dostupno: https://www.umj.com.ua/article/160341/novi-antibiotiki-mehanizmi-diyi-ta-rezistentnosti [in Ukrainian].
3. Hadzhula NH. Indyvidualʹnyy pidkhid do profilaktyky odontopatolohiyi u vahitnykh iz riznym rivnem kariyesrezystentnosti. Biomedytsynsʹka y biosotsialʹna antropolohiya [Internet]. 2015;24:173-5. Dostupno: https://bba-journal.com/index.php/journal/article/view/46 [in Ukrainian].
4. Kovalʹchuk VP, Isnyuk AS, Vovk IM, Kishchuk VV, Kovalenko IM. Porivnyalʹna kharakterystyka diyi pryrodnykh ta syntetychnykh antyseptychnykh preparativ na mikroorhanizmy, yaki zumovlyuyutʹ bakterialʹni infektsiyi verkhnikh dykhalʹnykh shlyakhiv. Visnyk vinnytsʹkoho natsionalʹnoho medychnoho universytetu. 2018;22(2):257-62. [in Ukrainian].
5. Ryabokonʹ EN. Éffektyvnostʹ prymenenyya preparatov rastytelʹnoho proyskhozhdenyya «Sandryn» y «Tymsal» v kompleksnom lechenyy zabolevanyy parodonta. Materialy mizhnarod. nauk.-prak. konfer. Nove ta tradytsiyne u doslidzhennyakh predstavnykiv medychnoyi nauky; 2014 Ber 28-2; Lʹviv, 2014. s. 45-7. [in Russiаn].
6. Bocharova VV. Іntegrated treatment of acne patients with peptide modulative and phytoantibacterial therapy. Dermatovenerology and cosmetology. 2018;2(3):52-71.
7.  Khin M, Jones AM, Cech NB, Caesar LK. Phytochemical Analysis and Antimicrobial Efficacy of Macleaya cordata against Extensively DrugResistant Staphylococcus aureus. Natural Product Communications. 2018;13(11):1479-83.
8. Normatyvno-dyrektyvni dokumenty MOZ Ukrayiny [Internet]. Nakaz MOZ Ukrayiny «Pro zatverdzhennya metodychnykh vkazivok «Vyznachennya chutlyvosti mikroorhanizmiv do antybakterialʹnykh preparativ». 2007 Kvit 5;167. Dostupno: http://mozdocs.kiev.ua/view. php?id=6958 [in Ukrainian].

«Bulletin of problems biology and medicine» Issue 1 (155), 2020 year, 274-277 pages, index UDK 615.322:616-022.6/7-08]-042.2

10.29254/2077-4214-2020-1-155-274-277