THE ROLE OF CREATININ AND UREA AS BIOMARKERS OF ORGANISM DAMAGE ON THE BACKGROUND OF ISCHEMIC REPERFUSION SYNDROME

Volotovska N. V.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Abstract. Goal. Establishing the dynamics of the concentration of creatinine and serum urea on the background of modifications of ischemic-reperfusion syndrome of the limb. Objects and methods. The study used 260 white adult male rats (200-250 g), which were divided into 5 groups: control, EG-1 – simulation of isolated ischemia-reperfusion (IR) of limb, EG-2 – simulation of isolated volumetric blood loss, EG-3 – combination of limb IR with blood loss, EG-4 – modeling of isolated mechanical injury of the thigh, EG-5 – combination of IR limb and mechanical injury. Serum creatinine and urea were determined in the serum after the experiment. Results and discussion. It has been established that 2-hour use of a tourniquet as an additional factor in blood loss or skeletal injury – impairs the excretory efficiency of the kidneys, especially, the level of creatinine and urea was statistically significantly increased in all study groups, compared with the control group. Comparing the severity of pathological changes between different groups, the features were: on the 7th day the creatinine content in EG-3 exceeded the data of EG-1 and EG-2 by 53.9% and 51.8%, appropriately. Also, it remained elevated, compared with EG-1 and EG-2 and on the 14th day – by 51.3% and 27%, appropriately. As for the combination of skeletal trauma with IR, on 3rd and 7th days there was a significant difference between the content of creatinine in EG-4 and EG-5. Thus, on the 3rd day the content in EG-5 was higher by 18.6%, and on the 7th day – by 13.8%. In the study of urea content, it was found that on the 7th day the indicator in EG-3 remained higher, compared with the data of EG-1 and EG-2 – in 2.5 times and by 39.7%, and on the 14th day was higher than them by 55.8% and 15.5% appropriately. Also, on the 7th day the indicator remained higher than the data of both groups – EG-1 and EG-4 – by 40.7% and by 44.2%. Conclusions. Each of the modifications of the limb IRS, including isolated ischemia-reperfusion, led to an increase in the studied parameters in the serum. The exception was an isolated injury.

Experiment, limb ischemia-reperfusion, creatinine, urea, kidneys, filtration, blood loss, skeletal trauma

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«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 30-35 pages, index UDK 616.61-008.8:547.495.2/.9:616.748-005.4]-092.9

10.29254/2077-4214-2021-1-159-30-35