INDUCED CHANGES IN THE CYTOLOGICAL PICTURE OF THE WOUND IN PATIENTS WITH PUTREFACTIVE-NECROTIC LESIONS OF THE DIABETIC FOOT SYNDROME WITH LOCAL APPLICATION OF VACUUM-INDUCED THERAPY

Kizimenko A. A., Lyakhovsky V. I., Grytsenko Ie. M., Zaopozhchenko A. V., Ivanov V. A., Uskov D. A.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Abstract. The content of our work is the study of the effectiveness of one of the variants of vacuum therapy, which combined the multiple positive effects of VAC therapy with programmed instillations of cooled and diluted povidone iodine in the treatment of purulent-necrotic lesions of the diabetic foot syndrome. The presented material of our article reflects the results of local cytological changes that arose under the influence of the proposed method among 55 patients, constituted the main study group, and also performed a detailed comparative cylotogic analysis with patients of the comparison group, which consisted of 52 patients and differed in local treatment in the form of gauze-ointment approach. For each patient, starting from day 1, and then on 4, 7, 10, smears of group prints were taken from the wounds using the method of “superficial biopsy” of the wound. The resulting material was transferred onto a glass slide, evenly distributed in a thin layer, fixed and stained according to the RomanovskyGiemsa method. Cytometric studies were carried out using the standard planes method and the average number of erythrocytes, altered neutrophils, naked neutrophils, macrophages, lymphocytes, fibroblasts, fibrin filaments and collagen fibers in the fields of vision was determined. According to the results obtained, the dynamics of changes in the phases of the wound process under the influence of vacuum therapy was monitored; it began on the 2nd day of hospitalization and lasted for 9 days. The induced changes in the cytological components of the wound recorded by us in the main group prove the effectiveness of our proposed technique, confirmed by the acceleration by 2 days of the transition of the first phase of the wound process to the second. This further has a positive effect on the final result of treatment, namely, a decrease in the percentage of complications and a reduction in the duration of inpatient treatment of such patients

diabetes mellitus, diabetic foot syndrome, povidone-iodine, vacuum therapy.

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«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 76-80 pages, index UDK 616.379-008.64-083-085

10.29254/2077-4214-2021-1-159-76-80