MICROSCOPIC CHANGES OF THE EXOCRINE PANCREAS AFTER EXPERIMENTAL THERMAL INJURY AND UNDER CONDITIONS OF APPLICATION OF LYOPHILIZED XENOGRAFT SKIN SUBSTRATE

Zykova N. P., Nebesna Z. M., Kramar S. B., Yakubyshyna L. V.

MORPHOLOGY

Scentific article

Abstract. Functional activity of practically all organs and organ systems in human body is affected at thermal trauma, in particular a pancreas. Therefore, in recent years in the treatment of burns are widely used tools that reduce the negative effects of toxins on the affected organism, in particular, the crushed substrate of lyophilized xenoskin. Therefore, the aim of the work was to establish microscopic changes of the pancreas exocrine part in the dynamics after experimental thermal trauma under the conditions of using the crushed substrate of lyophilized xenoskin. Grade III burn (20% of body surface) was applied with copper plates at temperature 97-100 ºC on the epilated back skin surface of 40 experimental mature white rats for 10 seconds under thiopental sodium anesthesia. 1 day after the burn, an early necrectomy of the damaged areas of the skin was performed. Then cleared areas were covered with a lyophilized xenoskin crushed substrate. Decapitation of animals was performed on 7, 14, 21 days of the experiment. The preparation of histological speciments was carried out according to conventional methods. Microscopic examinations of the pancreas exocrine part on the 7th day of the experiment, found that the lobular structure of the organ was preserved, however, there was swelling of the connective tissue stroma. The vast majority of exocrine pancreatocytes were hypertrophied. The nuclei were enlarged, their nucleoli were cleary visible. An increasing number of zymogen granules in the apical zone of cells caused the increasing of their secretory activity. Amoung unchanged exocrinocytes, destructively altered cells were found singly in the lobes of the gland. The vascular wall is characterized by moderate edema of adventitia, destruction of myocytes in tunica media and a decreasing leukocyte infiltration. The stasis of red blood cells in some hemocapillaries was detected. In the late stages of the experiment (14-21 days) there was a gradual normalization of the pancreas structural components. Conical pancreatocytes with enlarged round euchromatin nuclei and clear visible nucleoli were observed in the basal part and in the supranuclear part of the cell a large number of zymogen granules. The vessels were characterized by moderate blood supply, decreased wall edema, destruction of smooth arterial myocytes. Single leukocytes were detected in their adventitia. The veins and venules remained blood-filled. The vessels of the microcirculatory bed are moderately blood-filled without signs of slagging. Thus, the use of lyophilized xenoskin crushed substrate after thermal injury activates intracellular regeneration, which contributes to the normalization of the pancreas structural components.

pancreas, thermal injury, xenoskin.

1. Ahmad A, Herndon DN, Szabo C. Oxandrolone protects against the development of multiorgan failure, modulates the systemic inflammatory response and promotes wound healing during burn injury. Burns. 2019;45(3):671-681 DOI: 10.1016/j.burns.2018.10.006.
2. Elbassuoni EA, Abdel Hafez SM. Impact of chronic exercise on counteracting chronic stress-induced functional and morphological pancreatic changes in male albino rats. Cell Stress Chaperones. 2019;24(3):567-580 DOI: 10.1007/s12192-019-00988.
3. Greenhalgh DG. Management of Burns. New England Journal of Medicine. 2019;380(24):2349–2359. doi:10.1056/nejmra1807442.
4. Maden M. Optimal skin regeneration after full thickness thermal burn injury in the spiny mouse. Acomys cahirinus. Burns. 2018;44(6):1509- 1520. DOI: 10.1016/j.burns.2018.05.018.
5. Protsenko О, Shapoval O, Teslenko A, Voshylin B, Rodionov M, Yeletskyі N. Klinichni ta eksperymentalni doslidzhennia tkanyn pry termichnykh poshkodzhenniakh. Aktualni problemy suchasnoi medytsyny. 2019;3:4-13. DOI: https://doi.org/10.26565/2617- 409X-2019-3-01. [in Ukrainian].
6. Kearney L, Francis EC, Clover AJ. New technologies in global burn care - a review of recent advances. Int. J. Burns Trauma. 2018;8(4): 77-87.
7. Vons ВV, Chubka МB, Hroshovyі TA. Problema likuvannia opikovykh travm i kharakterystyka likarskykh zasobiv dlia mistsevoho likuvannia opikiv. Aktualni pytannia farmats i med nauky ta praktyky. 2018;11(1):119-125. DOI: 10.14739/2409-2932.2018.1.123731. [in Ukrainian].
8. Cherniakova HM, Minukhin VV, Voronin YeP. Suchasnyi pohliad na mistseve likuvannia opikiv z infektsiinoiu skladovoiu. Visnyk problem biolohii i medytsyny. 2016;1(4):68–72. [in Ukrainian].
9. Bigunyak VV, Povstianyi MYu, Volkov KS, Taran VM, Nahaichuk VI, Savchyn VS et al. Vykorystannia liofilizovanykh ksenodermotransplantantiv u kombustiolohii. Ternopil; 2003. 22 s. [in Ukrainian].
10. Horalskyi LP, Khomych VT, Kononskyi VT. Osnovy histolohichnoi tekhniky i morfofunktsionalni metody doslidzhen v normi ta pry patolohii. Zhytomyr: Polissya; 2015. 286 s. [in Ukrainian].
11. Kozhemiakin YuM, Khromov OS, Filonenko MA. Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy. Kyiv: Interservis; 2017. 179 s. [in Ukrainian].
12. Bilash SM, Pronina OM, Koptev MM. Comprehensive morphological studies as an intergal part of modern medical science. Literature review. Visnyk problem biolohiyi i medytsyny. 2019;2.2(151):20-3. DOI: 10.29254/2077-4214-2019-2-2-151-20-23.
13. Pronina OМ, Koptev MM, Bilash SМ, Yeroshenko GA. Response of hemomicrocirculatory bed of internal organs on various external factors exposure based on the morphological research data. Svit medytsyny ta biolohiyi. 2018;1(63):153-7. DOI: 10.26.724/2079-8334-2018-1- 63-153-157.

«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 209-212 pages, index UDK 611.37-091.8-02:616-001.17-085.361:599.731.1-035.51]-092.9

10.29254/2077-4214-2021-1-159-209-212