THE SUBSTANTIATION OF ANTIOXIDANT ACTION OF MEDICAMENTOUS COMPOSITION FOR TREATMENT OF PATIENTS WITH GENERALIZED PARODONTITIS AND PSYCHOSOMATIC STRESS

Kononova O. V.

DENTISTRY

Scentific article

Psychological stress is an important factor in the pathogenesis of periodontal disease. It is important to determine the relationship between psychosomatic stress and periodontal disease. For the treatment of stress in patients with generalized periodontitis, a medicinal composition is proposed. It is important to determine its antioxidant effect for the experimental substantiation of its periodontioprotective action. Objective:to determine the psychosomatic state of the inhabitants, the effect of psychological stress on the periodontal tissue and the experimental substantiation of the drug composition for the neutralization of the stress state. Methods:to study subjective human responses to the effect of various environmental factors a specially designed questionnaire is advisable to be used. To diagnosis of anxiety level was made by means of a self-determination test including reactive and personal anxiety according to Spielberger. Adrenaline stress was made in rats by oral application of gel with adrenalin in dose 0,36 mg/kg during 10 days. Lincomycin was introduced with drinking water in dose 60 mg/kg. The gel of adrenoblocators (zocson + nicergolin and sibason) was introduced by application in dose 0,6 mg/kg. The activity of catalase, contents of malonic dialdehide (MDA) were determined into serum and antioxidant-prooxidant index of API was calculated. Results.Testing by means of Spielberger test showed a moderate level of reactive anxiety among respondents –34.72±2.45 and a high level of personal anxiety –50.64±3.58. In patients with a high level of personal anxiety, a significantly higher prevalence of periodontal disease, especially generalized periodontitis, has been detected. Experimental studies have shown that gel adrenoblockers increases activity of catalase, reduces the content of MDA and normalizes the antioxidant-prooxidant index of AIP. Conclusions.A high level of personal anxiety –50.64±3.58 leads to a significant increase in the prevalence of periodontal diseases –95,09±6,7%, especially generalized periodontitis –88,72±6,5%. Oral application of the gel with adrenoblocators made an antioxidant effect after the modeling of adrenaline stress by administering animals to adrenalin and lincomycin.

reactive and personal anxiety of residents, periodontal diseases, adrenaline stress, adrenoblocator, antioxidant action

1. Astapov VM, redaktor. Trevoga i trevozhnost. SPb.: Piter; 2001. Razdel 3, Kontseptualnyie i metodologicheskie problemyi issledovaniya trivogi; s. 88-103. [in Russiаn].
2. Astapov VM, redaktor. Trevoga i trevozhnost. SPb.: Piter; 2001. Razdel 5, Funktsionalnyiy podhod k izucheniyu sostoyaniya trivogi; s. 156-65. [in Russiаn].
3. Borysenko AV, redaktor. Zakhvoriuvannia parodonta. Kyiv: Medytsyna; 2008. 614 s. [in Ukrainian].
4. Danilevskiy NF, Sidelnikova LF, Tkachenko AG. Rasprostranennost osnovnyih stomatologicheskih zabolevaniy i sostoyanie gigienyi polosti rta u naseleniya razlichnyih regionov Ukrainyi. Sovremennaya stomatologiya. 2006;2:14-16. [in Russiаn].
5. Ivanov VS, Barannikova IA, Balashov AN. Diagnostika sostoyaniya parodonta s ispolzovaniem standartnyih pokazateley (indeksov). Moskva; 1982. 21 s. [in Russiаn].
6. Kononova OV. Vliyanie linkomitsina na sostoyanie parodonta u kryis s adrenalinovyim stressom. Visnik stomatologiyi. 2016;3:26-28. [in Russiаn]
7. Kononova OV. Vliyanie oralnyih geley kvertulina i adrenoblokatorov na sostoyanie parodonta u kryis s adrenalinovyim stressom. Visnik stomatologiyi. 2016;4:8-11. [in Russiаn].
8. Kosenko KM. Epidemiolohiia osnovnykh stomatolohichnykh zakhvoriuvan u naselennia Ukrainy i shliakhy yikh profilaktyky [avtoreferat]. Kyiv: Ukrainskyi derzhavnyi medychnyi universytet; 1994. 45 s. [in Russiаn].
9. Levitskiy AP, Denga OV, Makarenko OA. Biohimicheskie markeryi vospaleniya tkaney rotovoy polosti. Metodicheskie rekomendatsii. Odessa; 2010. 16 s.[in Russiаn].
10. Mintser OP, Voronenko YuV, Vlasov VV. Obroblennia klinichnykh i eksperymentalnykh danykh u medytsyni. Kyiv: Vyshcha shkola; 2003. 350 s. [in Ukrainian].
11. Ostapko OI. Naukove obgruntuvannia shliakhiv ta metodiv profilaktyky osnovnykh stomatolohichnykh zakhvoriuvan u ditei v rehionakh z riznym rivnem zabrudnennia dovkillia [avtoreferat]. Kyiv: Nats. med. un-t; 2011. 38 s.[in Ukrainian].
12. Pavlenko OV, Antonenko MIu, Sidelnykov PV. Planuvannia likuvalno-profilaktychnoi dopomohy khvorym na heneralizovanyi parodontyt na osnovi otsinky ryzyku urazhennia parodontu. Sovremennaia stomatolohyia. 2009;1:56-61. [in Ukrainian].
13. Radyuk OM. Vosmifaktornyiy lichnostnyiy oprosnik Spilbergera-Radyuka. Minsk: RIVSh; 2009. 96 s. [in Russiаn].
14. Tarasenko LM. Patogenez povrezhdeniya parodonta pri stresse [avtoreferat]. Moskva: Moskovskiy med. stomatologicheskiy institut; 1986. 32 s.[in Russiаn].
15. Tarasenko LM, Petrushanko TA. Stress i parodont. Poltava; 1999. 192 s.[in Russiаn].
16. Tkachenko AH. Osoblyvosti klinichnoho perebihu, likuvannia ta profilaktyky heneralizovanoho parodontytu u osib molodoho viku 18-25 rokiv [avtoreferat]. Kyiv: Nats. med. un-t; 2006. 20 s. [in Ukrainian].
17. Truhacheva NV. Matematicheskaya statistika v mediko-biologicheskih issledovaniyah s primeneniem paketa Statistica. Moskva: GEOTARMedia; 2012. 379 s. [in Russiаn].
18. Chyzhevskyi IV. Klinichne ta hihiienichne obhruntuvannia profilaktyky kariiesu zubiv u ditei u promyslovo rozvynutomu rehioni [avtoreferat]. Kyiv: Nats. med. un-t; 2010. 38 s. [in Ukrainian].
19. Shorohov EV, redaktor. Sotsialnaya psihologiya i obschestvennaya praktika. Moskva: Nauka; 1985. Razdel 3, Lichnostnyie i sotsialno-psihologicheskie oprosniki v prikladnih issledovaniyah: problemyi i perspektivyi; s. 163-77. [in Russiаn].
20. Akhter R, Hannan M, Okhuba R, Morita M. Relationship between stress factor and periodontal disease in a rural area population in Japan. Eur. J. Med. Res. 2005;10(8):352-57.
21. Breivik T, Thrane PS. Psychoneuroimmunology. San Diego: Academic Press; 2001. Chapter 12, Psychoneuroimmune interaction in periodontal disease; p. 627-44.
22. Deinzer R, Granrath N, Spahl M, Linz S, Waschul B, Herforth A. Stress, oral health behavior and clinical outcome. Br. J. Health Psychol. 2005;10(2):269-83.
23. Omigbodun OO, Odukogbe AT, Omigbodun AO, Yusuf OB, Bella TT, Olayemi O. Stressors and physiological symptoms in students of medicine and allied health professions in Nigeria. Soc. Psychiatry Psychiatr. Epidemiol. 2006;41(5):15-421.
24. Parma C. Parodontopathien. Leipzig: I. A. Verlag; 1960. 203 p.
25. Peruzzo DC, Benatti BB, Ambrosano GM. A systematic review of stress and psychological factors as possible risk factors for periodontal disease. J. Periodontol. 2007;78(8):1491-1504.
26. Pistorius A, Krahwinkel T, Willerhausen B, Bockstegen C. Relationship between stress factors and periodontal disease. Eur. J. Med. Res. 2002;7(9):393-98.
27. Smith CK, Peterson DF, Degenhardt BF, Johnson JC. Depression, anxiety, and perceived hassels among entering medical students. Psychol. Health. Med. 2007;12(1):31-9.
28. Spielberger СD. Test Anxiety Inventory. Sampler Set. Manual, Test, Scoring. Redwood City: Mind Garden; 1980. 240 p.

«Bulletin of problems biology and medicine» Issue 1 Part 1 (142), 2018 year, 355-359 pages, index UDK 616.314. – 17-008.18-002

10.29254/2077-4214-2018-1-1-142-355-359