OBJECTIVATION OF CRITERIAL SIGNIFICANCE OF MYOCARDIAL TISSUE STRUCTURAL AND BIOCHEMICAL CHANGES IN FORENSIC DIAGNOSIS OF PRESCRIPTION OF DEATH COMING

Konoval N. S.

FORENSIC MEDICINE

Scentific article

Myocardial tissue of 30 dead bodies of people in early post-mortem period (within the time intervals of 3-13 hours since the death time) has been studied in research. The structural and biochemical markers in the myocardium homogenates have been assessed, namely, content of glycogen, acid phosphatase, lactate, lactate dehydrogenase, lipofuscin, cholinesterase. It was determined that structural and biochemical changes in myocardial tissue in the early postmortem period were characterized by typical morphological biochemical and biophysical changes, the main of which were: 1) gradual and constant decrease in relative optical density of nucleus and cytoplasm of cardiomyocytes within 3-13 hours since the death time; the rate and level of dynamics non-linearly depend on prescription of death coming; the quantitative dependencies (in the form of polynomials) regarding biophysical markers of morphological post-mortem changes of cardiomyocytes have been reasonably determined. Moreover, the comparative morphological study of cardiomyocytes ultrastructure in the early post-mortem period (in various time intervals depending on prescription of death coming) has been carried out; 2) statistically significant biochemical changes in the myocardial muscles were revealed in the early postmortem period, among which the most significant was a decrease in the glycogen content while simultaneously increasing the content of lipofuscin. The representative absolute and relative values of content for all six structural and biochemical markers were obtained depending on prescription of death coming; 3) correlation indicators between the biochemical and biophysical properties of muscle tissue of the myocardium were studied in the system interconnection, the corresponding systemic coefficients (for time intervals every 2 hours) were calculated, critically important structural and biochemical markers were substantiated to increase the accuracy of forensic diagnosis of prescription of death coming.

myocardium, structural-biochemical markers, post-mortal period, prescription of death coming, diagnosis, forensic medicine.

1.  Bachynskyi VT, Harazdiuk MS, Bezhenar IL, Kyshkan YaS. Mozhlyvist vyznachennia chasu nastannia smerti za vlasnoiu fluorestsentsiieiu plivok likvoru u korotkokhvylovomu spektri. Materialy naukovoi konferentsii profesorsko-vykladatskoho skladu VZO. Bukovynskyi derzhavnyi medychnyi universytet. Chernivtsi; 2017. s. 4-5. [in Ukrainian].
2.  Bachynskyi VT. Suchasni diahnostychni mozhlyvosti vyznachennia davnosti nastannia smerti: problemy ta perspektyvy. Tezy mizhnarodnoi konferentsii «Suchasni dosiahnennia v haluzi sudovoi medytsyny ta problemni pytannia pry provedenni sudovo-medychnykh ekspertyz u viddili komisiinykh ekspertyz ta u viddili ekspertyzy trupiv». Lviv; 2016. s. 302-4. [in Ukrainian].
3.  Olkhovskiy VO, Holubovych LL, redaktory. Vyznachennia davnosti nastannia smerti [monohrafiia]. Kharkiv: FOP Brovin OV; 2017. 168 s. [in Ukrainian].
4. Harazdiuk MS, Bachynskyi VT, Vanchuliak OIa, Ushenko OH. Vyznachennia davnosti nastannia smerti shliakhom dvomirnoho kartohrafuvannia poliaryzatsiino-neodnoridnykh zobrazhen polikrystalichnykh plivok likvoru. Klinichna ta eksperymentalna patolohiia. 2016;15(1):36-42. [in Ukrainian].
5.  Hladkykh DB. Sudovo-medychna diahnostyka davnosti nastannia smerti za supravitalnoiu reaktsiieiu zinyts [avtoreferat]. Kyiv; 2015. 19 s. [in Ukrainian].
6.  Hurov OM, Kozachenko IM, Kutsenko SV, Hladkykh DB. Vstanovlennia davnosti nastannia smerti u rannomu posmertnomu periodi za supravitalnoiu reaktsiieiu zinyts na diiu pilokarpin. Krymynalystyka y sudebnaia medytsyna: Mezhvedomstvennyi nauchno-metodycheskyi sbornyk. 2014;59:443-51. [in Ukrainian].
7. Kuzovkov AV, Vavylov AIu. Obyektyvyzatsyia dyahnostycheskykh alhorytmov ustanovlenyia davnosty smerty cheloveka po dynamyke tympanycheskoi temperatury. Sovremennye problemy nauky y obrazovanyia. 2017;2:12. [in Russian].
8. Olkhovskyi VO, Holubovych LL, Khyzhniak VV, Konoval NS. Vyznachennia tryvalosti posmertnoho periodu v sudovo-medychnii ekspertnii praktytsi: suchasnyi stan i perspektyvy. Eksperymentalna i klinichna medytsyna. 2016;4(73):154-62. [in Ukrainian].
9. Moskalenko VM, Voronenko YuV, redaktory. Sotsialna medytsyna ta orhanizatsiia okhorony zdorovia. Ternopil; 2002. s. 50-75. [in Ukrainian].

«Bulletin of problems biology and medicine» Issue 1 Part 2 (149), 2019 year, 385-389 pages, index UDK 340.6:[616/127:616.5]-091.1-91.8

10.29254/2077-4214-2019-1-2-149-385-389