THE PERSPECTIVES OF STUDYING OF QUALITATIVE AND QUANTITATIVE CHARACTERISTICS OF THE BONE TISSUE OF THE HUMAN FETUSES BY MEANS OF THE COMBINED AND HYBRID RESEARCH METHODS

Oshurko A. P., Oliinyk I. Yu., Gryshchuk G. V.

LITERATURE REVIEWS

Scentific article

Nowadays, scientific achievements very often require the determination of contents of micro- and macro elements, which cannot be defined by a certain method of analysis. Scientific progress provokes appearance of the necessity to select easy to execute, accurate, sensitive methodologies, which will give the opportunity to identify the component in complex biological mixtures. To solve this problem, we have selected the separation and concentration methods that give the opportunity to eliminate complex, hardly predictable situations to the large extent. Moreover, in many cases concentration broadens the borders of the use of instrumental control methods (atomic absorption spectrometry, spectrophotometry). Having analyzed the scientific resources with a choice of the combined and hybrid methods to perform the fragment of scientific research in terms of studying of the qualitative and quantitative characteristics of the upper jaw bone tissue of a human in dynamics of prenatal ontogenesis, we would like to emphasize that the atomic absorption spectrum is simpler than the emission, because it contains only the resonance lines and exactly those of them that have higher excitation potential than the emission ones. Absorption spectra are slightly shifted to a short-wave area. By the technique of the analysis, the atomic absorption photometry of the flame reminds the emission: the investigated solution is sprayed into the flame, where the processes of atomization and ionization are being performed, forming chemical compounds, and these processes are common for both variants of flame photometry; similar obstacles are also present during identification of the elements. However, it is necessary to mention that spectral barriers in atomic and absorption method are considerably smaller than in the emission, because absorption lines have small width and lamps with hollow cathode do not emit light, molecular lines of which would overlay onto these lines. The most important obstacle is non-selective (molecular) identification, which is pre-defined by the big concentration of the extraneous elements in complex, in our case, biological objects and systems, insufficient monochromatization of the light flux. In order to identify the concentration of the studied solution by means of the atomic and absorption photometry of the flame, the same methods as in the emission are used, namely – the method of the graded graph, comparison and standard addition method. Method of the graded graph is rationally to use in case of analysis of a large number of similar samples. By the comparison method, the magnitudes of the signals and the concentration of the studied and standard solutions are compared, this method is used when the qualitative composition of the samples is known and the determined area of linear dependence is defined. As of today, the atomic and absorption photometry method is used to identify more than 70 elements in different objects – biological solutions. Method is characterized by its expressiveness, selectivity and low limit of identification (up to 0,0005 mg/ml) as well as by its fairly high accuracy (1-5 %). Taking into account the studied scientific sources of literature, a considerable amount of available scientific research and our analysis, we can state that there is a good prospect for the growth of the use of combined and hybrid methods for determining the qualitative and quantitative characteristics of biological objects while conducting modern and up-to-date scientific research in morphology.

bone tissue, qualitative and quantitative characteristics, fetus, human

1. Moskvin LN, Caricyna LG. Metody razdelenija i koncentracii v analiticheskoj himii: monografija. Leningrad: Himija; 1991. 255 s. [in Russiаn].
2. Zolotov YuA, Kuzmin NM. Kontsentrirovaniye mikroelementov: monografiya. Moskva: Khimiya; 1982. 288 s. [in Russiаn].
3. Ponomarenko SI. Zviazok mizh parametramy stomatolohichnoho statusu ta systemnymy pokaznykamy stanu kistkovoi systemy. Problemy osteolohii. 2015;18(2):40-50. [in Ukrainian].
4. Khavezov I, Tsalev D. Atomno-absorbtsionnyy analiz. Moskva: Khimiya; 1983. 144 s. [in Russiаn].
5. Nabyvanets BI, Sukhan VV, Kalabina LV. Analitychna khimiia pryrodnoho seredovyshcha: pidruchnyk. Kyiv: Lybid; 1996. 304 s. [in Ukrainian].
6. Chmilenko FO, Sayevich OV. Sovremennyye tendentsii razvitiya mnogoelementnogo analiza biologicheskikh prob. Voprosy khimii i khimicheskoy tekhnologii. 2013;3:152-7. [in Russiаn].
7. Tarasova IV, Klymenko TM, Pohorielov M, Markevych VE, Sikora VZ, vynakhidnyky; Sumskyi derzhavnyi universytet, zaiavnyk i vlasnyk. Sposib kompleksnoho vyznachennia makro- ta mikroelementiv v orhanakh novonarodzhenykh laboratornykh shchuriv. Рatent Ukrainy 76421, MPK G01N 33/48 (2006.01) / zaiavka № u2012 05008; zaiavl. 23.04.2012; opubl. 10.01.2013. Biul. № 1. 4 s. [in Ukrainian].
8. Prays V. Analiticheskaya atomno-absorbtsionnaya spektroskopiya. Moskva: Mir; 1976. 358 s. [in Russiаn].
9. GOST 4011-72. Izdaniye. Voda pityevaya. Metody izmereniya massovoy kontsentratsii obshchego zheleza. [Data vvedeniya 1974-01-01; 201007-09 pereizd. s izm. 1]. Izd. ofits. Moskva: Standartinform; 2008. 8 s. Dostupno: http://vsegost.com/Catalog/26/26620.shtml [in Russiаn].
10. Dakhno L. Doslidzhennia shchilnosti kistkovoi tkanyny komirkovoho vidrostka verkhnoi shchelepy v osib riznoi stati y analiz yii vikovoi dynamiky. Pratsi NTSh. Med. nauky. 2015;XLI:64-72. [in Ukrainian].
11. Analytical Methods for Atomic Absorption Spectroscopy. The Perkin-Elmer Corporation; 1996. 300 p.
12. Gorsuch TT. The Destruction of Organic Matter: International Series of Monographs on Analytical Chemistry. Oxford: Pergamon Press; 1970. 160 p.
13. Busev AI, Simonova LN. Analiticheskaya khimiya sery. Moskva: Nauka; 1975. 272 s. [in Russiаn].
14. Krynytskyi RP. Analiz mineralnoho skladu kistkovoi tkanyny komirkovoi chastyny nyzhnoi shchelepy ta yoho vikovoi dynamiky u osib cholovichoi ta zhinochoi stati. Klinichna anatomiia ta operatyvna khirurhiia. 2015;14(3):40-3. [in Ukrainian].
15. Kramarenko VP. Toksykolohichna khimiia: pidruchnyk. Kyiv: Vyshcha shkola; 1995. 423 s. [in Ukrainian].
16. Subramanian KS. Determination of metals in biofluids and tissues: sample preparation methods for atomic spectroscopic techniques. Spectrochimica Acta Part B. 1996;51(3):291-319.
17. Kordiyak OY, Masna ZZ, Gryshchuk GV. Otsenka mineralnogo sostava kostnoy tkani alveolyarnogo otrostka nizhney chelyusti krys pri eksperimentalnom paradontite. Zdravookhraneniye. 2014;6:129-33. [in Russiаn].
18. Ferros IN, Mora MJ, Obeso IF, Jimenez P, Martinez-Insua A. The nasomaxillary complex and the cranial base in artificial cranial deformation: relationships from a geometric morphometric study. Eur. J. Orthod. 2015;37(4):403-11. DOI: 10.1093/ejo/cju066
19. PND F 14.1:2.112-97. Kolichestvennyy khimicheskiy analiz vod. Metodika vypolneniya izmereniy massovoy kontsentratsii fosfat-ionov v probakh prirodnykh i ochishchennykh stochnykh vod fotometricheskim metodom s vosstanovleniyem askorbinovoy kislotoy. Izd. ofits. Moskva: 1997 (pereizd. 2004). 18 s. Dostupno: http://docs.cntd.ru/document/1200056718 [in Russiаn].
20. Marchenko Z. Fotometricheskoye opredeleniye elementov. Per. s polskogo Matveyevoy IV, Nemodruka AA; pod red. Zolotova YuA. Moskva: Mir; 1971. 501 s. [in Russiаn].
21. Poluektov NS. Metody analiza po fotometrii plameni. Moskva: Khimiya; 1967. 307 s. [in Russiаn].
22. Braynina KhZ, Neyman EYa, Slepushkin VV. Inversionnyye elektroanaliticheskiye metody. Moskva: Khimiya; 1988. 240 s. [in Russiаn].
23. Vydra F, Shtulik K, Yulakova E. Inversionnaya voltamperometriya. Рer. s cheshskogo Nemova VA; pod. red. Kaplana BYa. Moskva: Mir; 1980. 279 s. [in Russiаn].
24. Khentse G. Polyarografiya i voltamperometriya. Teoreticheskiye osnovy i analiticheskaya praktika. Рer. s nem. Garmasha AV, Kameneva AI. Moskva: BINOM. Laboratoriya znaniy; 2011. s. 172-244. [in Russiаn].
25. Tsyhykalo OV, Popova IS, Kuznyak NB, Palis SYu, Shostenko AA, Dronyk II. Suchasni uiavlennia pro patohenez pryrodzhenykh vad lytsia (ohliad literatury). Bukovynskyi medychnyi visnyk. 2017;21(1):230-4. [in Ukrainian].
26. Pechalova PF, Poriazova EG, Pavlov NV. Residual cyst of the jaws. Curierul medical. 2011;5(323):15-7.
27. Oshurko AP, Oliinyk IIu. Morfohenez verkhnoi shchelepy ta okremykh struktur shchelepno-lytsevoi dilianky v zarodkovomu periodi prenatalnoho ontohenezu liudyny. Klinichna ta eksperymentalna patolohiia. 2017;XVI(2)2:137-44. [in Ukrainian].
28. Oshurko AP, Oliinyk IYu. Innovation in the study of the features of the structure of the human upper jaw in the dynamics of prenatal ontogenesis. In: Materials of the 98th final scientific conference of the teaching staff of the Higher State Educational Institution of Ukraine «Bukovinian State Medical University»; 2017 February 13, 15, 20; Chernivtsi, Ukrainе. Chernivtsi: Meduniversytet; 2017. р. 19-20.
29. Akhtemiichuk YuT, redactor. Narysy perynatalnoi anatomii. Chernivtsi: BDMU; 2011. 300 s. [in Ukrainian].
30. Piskunov IS, Piskunov VS, Bobryshev SV, Kononenko NI. Frontonazalnaya displaziya i ageneziya verkhnechelyustnykh pazukh. Rossiyskiy elektronnyy zhurnal radiologii. 2014;4(2):127-30. Dostupno: www.rejr.ru [in Russiаn].
31. Dmytrenko MI. Osoblyvosti kompleksnoi diahnostyky patsiientiv iz zuboshchelepnymy anomaliiamy, uskladnenymy skupchenistiu zubiv. Visnyk problem biolohii i medytsyny. 2016;1(2):232-5. [in Ukrainian].
32. Tsyhykalo OV, Popova IS, Shostenko AA, Dronyk II, Perebyinis PP. Morfolohichni osoblyvosti dilianok i trykutnykiv shyi liudyny. Visnyk problem biolohii i medytsyny. 2016;4(2):26-31. [in Ukrainian].
33. Kryvetskyi VV, Protsak TV, Haina NI, Kozar OM. Morfohenez verkhnoshchelepnoi pazukhy v ontohenezi liudyny. Molodyi vchenyi. Lystopad 2015;11(26)3:84-7. [in Ukrainian].
34. Tereshchenko VA, Stasova YuV. Mandibulo-fatsialnyy dizostoz (sindrom Franchesketti) u novorozhdennogo: opisaniye klinicheskogo sluchaya. Byulleten meditsinskikh internet-konferentsiy (ISSN 2224-6150). 2015;5(5):360-1. ID: 2015-05-376-A-4929. Dostupno: www.medconfer.com [in Russiаn].
35. Kuzniak NB. Zakonomirnosti indyvidualnoi anatomichnoi minlyvosti nosovoi dilianky u plodiv liudyny. ISSN 1681-276X. Visnyk naukovykh doslidzhen. 2016;4:35-40. [in Ukrainian].
36. Tsyhykalo O, Kuzniak N, Popova I, Oliinyk IYu, Dmytrenko RR, Perebyinis PP, et al. Peculiarities of the upper lip morphogenesis and its relation to facial development (review). Galician medical journal. 2017;24(2):1-4. E2017217. DOI: 10.21802/gmj.2017.2.17
37. Mishalov VD, Chaikovskyi YuB, Tverdokhlib IV. Dotrymannia etychnykh i zakonodavchykh norm i vymoh pry vykonanni naukovykh morfolohichnykh doslidzhen. Kyiv; 2007. 76 s. [in Ukrainian].
38. Mishalov VD, Voichenko VV, Malysheva TA, Dibrova VA, Kuzyk PV, Yurchenko VT. Poriadok vyluchennia biolohichnykh obiektiv vid pomerlykh, tila yakykh pidliahaiut sudovo-medychnii ekspertyzi i patolohoanatomichnomu doslidzhenniu, dlia naukovykh tsilei: metodychni rekomendatsii. Osvita Ukrainy: spetsvypusk hazety. Kyiv: Pedahohichna presa. 2018;2(62):3-13. [in Ukrainian].
39. Abadzhev SS, Semenyshyn DI, Yatchyshyn YI, Borova OIa, Tsiupko FI, Marshalok HO, ta in. Fizyko-khimichni (instrumentalni) metodi analizu: konspekt lektsii z kursu «Analitychna khimiia» dlia studentiv khimichnykh spetsialnostei. Chastyna 2. Lviv: Vydavnytstvo Natsionalnoho universytetu «Lvivska politekhnika»; 2004. 116 s. [in Ukrainian].
40. Zolotov YuA, redactor. Osnovy analiticheskoy khimii. V 2-kh knigakh. Kniga 2. Metody khimicheskogo analiza: uchebn. dlya vuzov; Seriya «Klassicheskiy universitetskiy uchebnik». 3-e izd. pererab. i dop. Moskva: Vysshaya shkola; 2004. 503 s. [in Russiаn].
41. Kalvoda R, Zyka Ya, Shtulik K, i dr. Elektroanaliticheskiye metody v kontrole okruzhayushchey sredy. Рer. s angl. pod red. Neymana EYa. Moskva: Khimiya; 1990. 240 s. [in Russiаn].

«Bulletin of problems biology and medicine» Issue 2 (144), 2018 year, 43-49 pages, index UDK 611.018.4-053.18:57.086

10.29254/2077-4214-2018-2-144-43-49