SUSCEPTIBILITY TO ANTIBIOTICS OF STAPHYLOCOCCUS AUREUS STRAINS, ISOLATED FROM UPPER RESPIRATORY TRACT OF HUMAN

Vashchenko A. O., Valchuk S. I., Voronkova Yu. S., Shevchenko T. M., Voronkova O. S.

MICROBIOLOGY

Scentific article

Abstract. The aim of the research. To investigate the frequency of detection of staphylococci of different species in the microbiota of the human upper respiratory tract and to study the resistance to antibiotics of isolated strains.Object and methods of the research. The object of the research was antibiotic resistance of biofilm forming strains of staphylococci isolated from the nasal cavity of persons with non-allergic rhinitis. Microscopic and bacteriological methods were used to isolate and identify the staphylococci. The study of the ability to form a biofilm was performed on 96-well plastic plates. Antibiotic susceptibility was determined according to CLSI standards. The results of the study and their discussion. It was found that the most common manifestations of non-allergic rhinitis in the microbiota of the nasal cavity are: S. aureus – 64.4% (31), S. epidermidis – 18.8% (9), S. saprophyticus – 10.4% (5) and Staphylococcus spp. – 6.3% (3). 87.5% (42) of the isolated strains of staphylococci were able to form a biofilm after 72 h of cultivation. Of these: all strains of S. epidermidis (9), 90.3% (28) – S. aureus, 80% (4) – S. saprophyticus and 1 – Staphylococcus spp. It was found that more than 50% were resistant to doxycycline. For other antibiotics, the best effect was found for meropenem, to which only 21.4% (6) and 33.3% (3) of S. aureus and S. epidermidis strains were resistant, respectively. Conclusions. The predominance of S. aureus strains among other staphylococci in the microbiota of the nasal cavity in persons with non-allergic rhinitis: 64.6% (31) cases. It was determined that most strains of staphylococci of different species have a tendency to form a biofilm: 87.5% (42) of cases. It has been determined that azithromycin, amoxicillin / clavulanic acid and ofloxacin may be recommended as drugs of choice for the treatment of rhinitis in surveyed contingent. The reserve drug is meropenem, and doxycycline can be used only in determining the sensitivity to it in a particular strain.

staphylococci, nasal cavity, non-allergic rhinitis, biofilm, antibioticresistance.

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«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 177-180 pages, index UDK 579.864:616.1

10.29254/2077-4214-2021-2-160-177-180