MICROBIOLOGICAL REASONING OF N-CHLOROTAURINE USE IN SECONDARY PERITONITIS

Kusminyh S. S., Ishchenko O. V., Stetsenko I. Yu., Titov G. I., Makarenko O. V.

MICROBIOLOGY

Scentific article

Abstract. Introduction. Microbiological assay with determination of individual antimicrobial susceptibility is one of the necessary diagnostic methods for peritonitis of any origin. The use of N-chlorotaurine in the treatment ofbacterial, viral, and protozoal infections of various origins is widely discussed nowadays. The study aimed to assess the microbial spectrum of causative agents of secondary peritonitis, its sensitivity to antimicrobial drugs and Nchlorotaurine. Materials and methods. The study included adults aged 18 to 65 years who had secondary peritonitis associated with acute surgical diseases of the gastrointestinal tract. Determination of the microbial profile of peritoneal exudate was performed by the culture method. The profile of chemotherapeutic sensitivity was determined by the disc diffusion method. The antimicrobial activity of N-chlorotaurine was determined by culturing the obtained isolates in a solution with pH 5.0 and 7.0. Postantibiotic effect was defined as indicators of growth recovery after sublethal contact with the solution of the test antiseptic. Results of the research. Among the etiological factors of secondary peritonitis, the biggest proportion comprised the Enterobacteriaceae family, namely Escherichia coli (n=26; 36.11%). The sensitivity of the obtained cultures to antimicrobial drugs was variable. There were 41.17 % of producers of extended-spectrum beta-lactamases were among the Enterobacteriaceae family; the cultures were obtained from 12 (27.27%) participants. A 1% solution of N-chlorotaurine showed significant bactericidal activity against all experimental cultures. Antimicrobial activity and postantibiotic effect were potentiated by acidic pH. At pH 7.0, all test cultures, namely Pseudomonas aeruginosa, Escherichia coli, Klebsiella oxytoca, were less susceptible than gram-positive microorganisms, particularly Staphylococcus aureus, and Proteus mirabilis. After sublethal contact, chemotherapeutic sensitivity was improved statistically significantly. Conclusions. N-chlorotaurine is a promising antiseptic for use in the treatment of secondary peritonitis.

N-chlorotaurine, secondary peritonitis, ESBL.

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«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 184-188 pages, index UDK 616.381-002-021.633-085-036.5:615.28

10.29254/2077-4214-2021-2-160-184-188