FEATURES OF REMODELING OF VENOUSVESSELS OF VENTRICULESHEART AT POSTRESECTION PORTAL HYPERTENSION

Hnatjuk M. S, Gdanska N. M, Tatarchuk L. V, Yasinovsky O. B.

MORPHOLOGY

Scentific article

Abstract. It is known that the structural and functional reconstruction of arterial and venous vessels of the heart can be caused by various changes in hemodynamics in the great and small circles of blood circulation and the system of the hepatic portal vein. It should be noted that the venous vessels of the ventricles of the heart in postresection portal hypertension are insufficiently studied. The purpose of research to study the peculiarities of the structural reoconstruction of the venous bad of the ventricles of the heart at postresection portal hypertension by quantitative morphological methods. Methods and Material. The venous bad of the ventricles of the heart of 54 adult white male rats, which were divided into 3 groups, was morphometrically studied. 1 group – 15 intact animals, 2 – 28 rats, which simulated postresection portal hypertension by removing the left and right lateral lobes of the liver, 3 – 11 animals with postresection portal hypertension and multiorgan failure. Thirty days after the start of the experiment, euthanasia of experimental animals was performed by bloodletting under conditions of thiopental anesthesia. On histological micropreparations of the left and right ventricles determined the diameters of capillary venules, venules, external and internal, the wall thickness of venous vessels, the height of endotheliocytes, their nuclei, nuclear-cytoplasmic relations in these cells, the relative volumes of damaged endothelial cells. Quantitative indicators were processed statistically. Results and Discussion. A complecsive analysis of the obtained morphometric parameters revealed that the most pronounced structural reconstruction of the venous vessels of the ventricles of the heart was observed in the combination of portal hypertension with polyorgan failure. So in the left ventricle, the diameter of the capillary venules statistically significantly (p<0.001) increased by 15.4%, venules – by 17.2% (p<0.001) compared with controls, in the right ventricle, respectively – by 11.0% and 14, 2% (p<0.001). There was a marked increase in the inner and outer diameters of the veins, a decrease in the thickness of the venous wall in the left ventricle by 22.8% (p<0.001), in the right – by 21.9% (p<0.001). Uneven, disproportionate changes in the morphometric characteristics of the nucleus and cytoplasm of venous endothelial cells led to violations of nuclear-cytoplasmic relations in these cells, which increased by 8.1% in the left ventricle and 5.2% in the right ventricle compared to the control. The relative volumes of damaged endothelial cells in the left ventricle statistically significantly (p<0.001) increased 17.6 times, in the right – 16.7 times. The density of microvessels in the left ventricle decreased by 7.9% (p<0.01 ), in the right – by 6.4% (p<0.01), which indicated a violation of microhemocirculation. The results show that postresection portal hypertension leads to a pronounced structural reconstruction of the venous bad of the ventricles of the heart, which significantly disrupts the drainage of venous blood from them and leads to hypoxia, deterioration of trophic supply, dystrophy, necrobiosis of cells and tissues, and cells role in the pathomorphogenesis of their lesions. The degree of remodeling of the venous bad was dominant in the left ventricle and in conditions of multiorgan failure.

postresection portal hypertension, venous bad of the ventricles of the heart.

1. Vishnievskiy VA, Yefanov MH, Kazakov YV. Siegmientarnye rieziektsyi, otdaliennye rezultaty pri zlokachestviennykh opukholiakh pecheni. Ukrayinskyy zhurnal khirurhiyi. 2012;1(16): 5-15. [in Ukrainian].
2. Коtеnkо ОH, Pоpоv АО, Hrynеnkо АV. Аgresivnaia technika khirurhicheskoho lechenia perichiliarnoi kholanhiokartsinomy s invaziei v vorotnu venu. Ukrainskyj zhurnal khirurhyi. 2018;1(36):53-57. [in Ukrainian].
3. Shin GH, Kim SU, Park JY. Liver stiffness-bases model for prediction of hepatocellular carcinoma in chronic hepatitis B virus infection: comparison with hisiological fibrosis. Liver Int. 2014;14:2133-2136.
4. Bosh J, Graszmann R, Shah V. Evalution in the understanding of the pathophysiological basis of portal hypertеnsion: how changes in paradigm are leading to success ful new treatments. J Hepatol. 2015;62(1):121-130.
5. Hnаtjuk МS, Tатаrchuk LV. Pоrushеnnia vsmoktuvаlnоi funktsii tоnkоi kyshky pry postrezektsijnii portalnoy hipertenziy. Visnyk naukovykh doslidzhen. 2018;2:115-120. [inUkrainian].
6. Dzyhal OF. Formuvannya polisyndromnoyi nedostatnosti khvorykh na tsyroz pechinky z portalnoyu hipertenziyeyu. Visnyk naukovykh doslidzhen. 2017;2: 88-92. [in Ukrainian].
7. Avtandilov GG. Osnovy kolichestviennoy patologicheskoy anatomii. Moskva: Meditsyna, 2002. 2403 s. [in Russian].
8. Baibakov VM. Morfofunktsionalni zminy venoznoho rusla yak lanky drenazhnoi systemy yayechka pry travmuvanni sudynnykh anastomoziv simyanoho kanatyka v eksperymenti. Klinichna anatomiia ta operatyvna khirurgiya. 2011;10(4):32-5. [in Ukrainian].
9. Pronina OМ, Koptev MM, Bilash SМ, Yeroshenko GA. Response of hemomicrocirculatory bed of internal organs on various external factors exposure based on the morphological research data. Svit medytsyny ta biolohiyi. 2018;1(63):153-7. DOI: 10.26.724/2079-8334-2018-1- 63-153-157.
10. Horalʹskyy LP, Khomych VP, Konopsʹkyy OI. Osnovy histolohichnoyi tekhniky i morfofunktsionalʹni metody doslidzhennya u normi ta pry patolohiyi. Zhytomyr: Polissya; 2011. 288 s. [in Ukrainian].
11. Bilash SM, Pronina OM, Koptev MM. Comprehensive morphological studies as an intergal part of modern medical science. Literature review. Visnyk problem biolohiyi i medytsyny. 2019;2.2(151):20-3. DOI: 10.29254/2077-4214-2019-2-2-151-20-23.
12. Grzhibovsky AI, Ivanov OV, Gorbatova MA. Sravnennia kolichestvennych dannych dvuch parnych vyborok s ispolzovanijm programnogo obespechenia Statistica i SPSS; parametricheskie and neparametricheski criterii. Nauka i zdrovokhranenie. 2016;3:5-25. [in Russian].
13. Reznikov OH. Zahalni etychni pryntsypy eksperymentiv na tvarynakh. Endokrynolohiia. 2003;8(1):142-145. [in Ukrainian].
14. Villnuela C, Albillas P, Genesca J. Development of hyperdynamic circulation and response to beta-blockers in compensaten cirrhosis with portal hypertension. Hepatology. 2016;63(1):197-206.

«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 206-209 pages, index UDK 616.36-089.87-06:616-091]-092.9

10.29254/2077-4214-2021-2-160-206-209