MORPHOMETRIC CHARACTERISTICS OF LYMPHATIC SINUS OF ILIAC LYMPH NODES OF WHITE RATS-MALS IN CONDITIONS OF LONG-TERM OPIOID NALBUPHINE INFLUENCE

Golovatsky A. S., Valko O. O., Kochmar M. Y.

MORPHOLOGY

Scentific article

The article presents the data on changes in the relative lymphatic sinuses of the cortical and cerebral substances of the ideal lymph nodes of white rats-male of reproductive age over a long (six-week) exposure of their organism to the opioid nalbuphine. The experimental research has been conducted on 52 outbred white male rats of reproductive age – 1.5 months old. All experimental animals were divided into 8 experimental groups: 1 group – 5 intact rats; group 2 – 5 rats with the nalbuphine administered daily for one week at a dose of 8 mg/kg; group 3 – 5 subjects treated with nalbuphine at a dose of 15 mg/kg during the second week; group 4 – 5 animals treated with nalbuphine for 20 mg/kg during the third week; group 5 – 5 subjects with the nalbuphine administered during the fourth week at a dose of 25 mg/ kg; group 6 – 5 animals treated with nalbuphine for 5 weeks at a dose of 30 mg/kg; group 7 – 5 rats with the nalbuphine administered during the sixth week at a dose of 35 mg/kg; group 8 – 5 individuals – one week after drug withdrawal. Nalbufin was administered intramuscularly daily. The experiments on the animals have been carried out in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986), and Council of Europe Directives 86/609/EEC (1986). The relative areas of cortical and cerebral substances were determined on histological preparations with thickness of 5–7 μm, stained with hematoxylin and eosin with the help of the morphometric method. The relative areas of the marginal lymphatic sinus, cortical and cerebral intermediate lymphatic sinuses of the iliac lymphoid lymph nodes were revealed using a system of visual analysis of histological preparations. Morphometric studies were carried out using VideoTest-5.0, CARRA Image Base, and Microsoft Exel on the computer. Statistical processing of digital data was performed using Exel and STATISTICA 6.0 software applying the parametric method. The mean probability (p) was determined by the Student’s t-test with a probability level of p <0.05. Having studied the lymph nodes of white male rats of reproductive age with a long-term (six weeks) exposure of the animal body to opioid nalbuphine, it has been found out that there occured significant changes in the relative area of the cerebellum (p <0.05) under the action of nalbuphine. In a week of the experiment, it significantly decreased (p <0.05) by 33.3 % compared to animals in the intact group (21.8 ± 1.07) %) and reached (14.52 ± 1.12) %. This indicator gradually decreases and after four weeks of the experiment reaches (11,96 ± 0,83) %, which is by 45,1% less than the index of animals in the intact group and is minimal during the whole experiment. In the future, this parameter slightly increases compared to the previous groups (14.57 ± 1.05) – after five weeks of exposure to nalbuphine), but does not correspond to the norm till the end of the experiment, remaining valid (p <0.05) less by 31.4% and is (14.95 ± 1.11) %. The relative areas of the marginal and cortical intermediate lymphatic sinuses has remained almost unchanged during the experiment. Their indicators fluctuated within the animals of the intact group.

lymph node, sinus lymph node, sinus apparatus, exposure, narcotic analgesic nalbuphine, rat

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«Bulletin of problems biology and medicine» Issue 3 (152), 2019 year, 286-291 pages, index UDK 611.428.061.1:615.212.7] – 092.

10.29254/2077-4214-2019-3-152-286-291