ANALYSIS OF HAND ECZEMA DEVELOPMENT DEPENDING ON GLUCOCORTICOID RECEPTOR GENE BclI POLYMORPHISM
About the author:
Methkal A. M., Kuts L. V.
Type of article:
Hand eczema is one of the most common chronic inflammatory skin and socially significant diseases which negatively affects the life quality and leads to disability and economic burden on society. The pathogenesis of hand eczema is multifactorial. Numerous studies indicate the complex nature of disorders from many body systems in patients with eczema, but the leading role belongs to genetically determined or acquired immune disorders. The glucocorticoid receptor plays an important regulatory role in the immune system and the development of many immune-dependent diseases. Since the differences in GR sensitivity may affect the effector immune functions and the development of immune-dependent diseases, the aim of this study was to determine whether there is a relationship between the development of hand eczema and BclI single nucleotide polymorphism of the glucocorticoid receptor gene. The venous blood of 143 patients with hand eczema (42% of women and 58% of men) age of (42.2 ± 11.1) years was used in the study. The control group was consisted of 97 people (50.5% women and 49.5% men) age of (43.8 ± 11.5) years. Single nucleotide polymorphism BclI (reference number – rs41423247) of the glucocorticoid receptor gene GR (NR3C1) was determined by the method described previously (Fleury et al. 2003). Statistical analysis was performed using the program SPSS 22.0. Quantitative variables were tested for normal distribution by the Shapiro-Wilk method. The comparisons of the means between the two groups were performed using Student’s t-test for independent samples. The frequency comparison of different indicators distribution between the control and the group of patients was performed using the Pearson’s test. Binary logistic regression was used to analyze the relationship between BclI polymorphism and the occurrence of hand eczema in different models. The P value <0.05 was considered significant. Thus, there was found that a statistically significant relationship exists in the dominant model (P = 0.016) during the study of the association between the development of hand eczema and NR3C1 gene BclI polymorphism. The risk of hand eczema in carriers of the minor allele G is 1.95 times higher (CI 95 % = 1.13 – 3.37) than in homozygotes of main allele. The association of BclI single nucleotide polymorphism with the occurrence of eczema under the recessive and superdominant model was not detected (P > 0.05).
BclI polymorphism, glucocorticoid receptor, hand eczema.
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Publication of the article:
«Bulletin of problems biology and medicine» Issue 3 (157), 2020 year, 174-177 pages, index UDK 616.5-002.2-616.5-002.158.091