HISTOMORPHOMETRIC CHARACTERISTICS OF RATS SKELETAL MUSCLES REGENERATION UNDER THE INFLUENCE OF CHRONIC HYPERGLYCEMIA
About the author:
Rtail R. A., Maksimova O. S., Berchanov O. M., Tkach G. F.
Type of article:
Chronic hyperglycemia (CH) is one of the most common metabolic disorders worldwide, and the number of people suffering from excess blood glucose continues to increase every year. CH is often associated with the development of secondary complications in various organs, including skeletal muscle. However, to date, there are practically no works devoted to establishing the structural features of skeletal muscle regeneration after their mechanical traumatization under the condition of CH influence on organism. The aim of the study was to analyze the histological and histomorphometric features of skeletal muscle posttraumatic recovery in rats with CH. Object and methods. 90 laboratory white male rats were used in the study: 40 rats – control group; 40 rats – experimental group (animals with simulated HG); 10 rats – hyperglycemia control group. Simulation of CH was carried out using a two-week loading with 10 % fructose solution, followed by a single intraperitoneal administration of streptozotocin (40 mg/kg). Triceps surae muscle mechanical injury was reproduced by linear deep incision perpendicular to the muscle fibers course, followed by comparison and stitching of the wound edges. The morphological features of skeletal muscle regeneration were studied at 3, 7, 14 and 28 days after mechanical injury. Light microscopy was performed using an Olympus BH-2 microscope (Japan). Statistical analysis was performed using the SPSS-17 software package. Results. On the 28th day of the experiment, the number of regenerating muscle fibers in the regeneration zone of experimental animals was smaller by 26.8% (P < 0.001 – for the pericentral zone) and 15.4 % (P < 0.001 – for the marginal zone), compared to control rats. The total area of regenerating muscle fibers (P < 0.001) in experimental group was also significantly smaller, compared to control animals. Also, rats with excessive glucose content had significant development of connective tissue elements, delayed formation of new blood vessels, accumulation of adipocytes, and persistence of leukocyte cells. Conclusion. Thus, the main structural manifestations of skeletal muscle post-traumatic regeneration process in rats with CH are incomplete myogenesis, excessive connective tissue development, new vessels formation disorders, reduction of regenerating muscle fibers amount and area along with significant increase of damaged muscle fibers number
skeletal muscles, regeneration, chronic hyperglycemia, mechanical trauma
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Publication of the article:
«Bulletin of problems biology and medicine» Issue 4 Part 2 (154), 2019 year, 312-316 pages, index UDK 616.24-018-06:577.118:613.32(043.5)