РЕПРОДУКЦІЯ І ФРАГМЕНТАЦІЯ ДНК СПЕРМАТОЗОЇДІВ

Калейнікова О. М., Срібна В. О., Виноградова-Аник О. О., Вознесенська Т. Ю., Блашків Т. В.

ОГЛЯДИ ЛІТЕРАТУРИ

Наукова стаття

Метою роботи став пошук і аналіз даних літератури про фрагментацію ДНК сперматозоїдів і її роль у репродукції.Факт зниження фертильності, ефективності методів допоміжних репродуктивних технологій і підвищення ризику вроджених вад багато дослідників пояснюють збільшенням рівня фрагментації ДНК сперматозоїдів. Проте, на сьогодні відсутній консенсус щодо клінічно значущих стандартів визначення величин фрагментації ДНК сперматозоїдів і її порогових рівнів. Не до кінця ясно, чи взаємопов’язані і як такі показники стандартної спермограми як концентрація, рухливість, морфологія сперматозоїдів і аномалії їх хроматину. Роль порушень просторової структури хроматину сперматозоїдів в репродукції потребує подальшого вивчення.

фертильність, фрагментація ДНК, сперматозоїд.

1. Simon L, Castillo J, Oliva R, Lewis S. Relationships between human sperm protamines, DNA damage and assisted reproduction outcomes. Reprod Biomed Online. 2011;23(6):724-34. DOI: 10.1016/j.rbmo.2011.08.010
2. Simon L, Murphy K, Shamsi M, Liu L, Emery B, Aston K, et al. Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod. 2014;29(11):2402-12. DOI: 10.1093/humrep/deu228
3. Zini A, Meriano J, Kader K, Jarvi K, Laskin C, Cadesky K. Potential adverse effect of sperm DNA damage on embryo quality after ICSI. Hum Reprod. 2005;20(12):3476-80.
4. Sigman M. Testicular versus ejaculated sperm should be used for intracytoplasmic sperm injection (ICSI) in cases of infertility associated with sperm DNA fragmentation. Int Braz J Urol. 2018;44(4):676-9. DOI: 10.1590/S1677-5538.IBJU.2018.04.04
5. Arafa M, AlMalki A, AlBadr M, Burjaq H, Majzoub A, AlSaid S, et al. ICSI outcome in patients with high DNA fragmentation: Testicular versus ejaculated spermatozoa. Andrologia. 2018;50(1). DOI: 10.1111/and.12835.
6. Irvine D, Twigg J, Gordon E, Fulton N, Milne P, Aitken R. DNA integrity in human spermatozoa: relationships with semen quality. J Androl. 2000;21(1):33-44.
7. Mantas D, Angelopoulou R, Msaouel P, Plastira K. Evaluation of sperm chromatin quality and screening of Y chromosome microdeletions in Greek males with severe oligozoospermia. Arch Androl. 2007;53(1):5-8.
8. Santi D, Spaggiari G, Simoni M. Sperm DNA fragmentation index as a promising predictive tool for male infertility diagnosis and treatment management – meta-analyses. Reprod Biomed Online. 2018;37(3):315-26. DOI: 10.1016/j.rbmo.2018.06.023
9. Virro M, Larson-Cook K, Evenson D. Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril. 2004;81:1289-95.
10. Ozmen B, Koutlaki N, Youssry M, Diedrich K, Al-Hasani S. DNA damage of human spermatozoa in assisted reproduction: origins, diagnosis, impacts and safety. Reprod Biomed Online. 2007;14(3):384-95.
11. Małgorzata K, Depa-Martynów M, Butowska W, Filipiak K, Pawelczyk L, Jedrzejczak P. Human spermatozoa ultrastructure assessment in the infertility treatment by assisted reproduction technique. Arch Androl. 2007;53(6):297-302.
12. Franco J, Mauri A, Petersen C, Massaro F, Silva L, Felipe V, et al. Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl. 2012 Feb;35(1):46-51. DOI: 10.1111/j.1365-2605.2011.01154.x
13. Watanabe S, Tanaka A, Fujii S, Mizunuma H, Fukui A, Fukuhara R, et al. An investigation of the potential effect of vacuoles in human sperm on DNA damage using a chromosome assay and the TUNEL assay. Hum Reprod. 2011;26(5):978-86. DOI: 10.1093/humrep/der047
14. Bragina EE, Bocharova EN. Kolichestvennoe elektronno-mikroskopicheskoe issledovanie spermatozoidov pri diagnostike muzhskogo besplodiya. Andrologiya i genitalnaya hirurgiya. 2014;1:41-50. [in Russian].
15. Kennedy C, Ahlering P, Rodriguez H, Levy S, Sutovsky P. Sperm chromatin structure correlates with spontaneous abortion and multiple pregnancy rates in assisted reproduction. Reprod Biomed Online. 2011;22(3):272-6. DOI: 10.1016/j.rbmo.2010.11.020
16. Simon L, Lutton D, McManus J, Lewis S. Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success. Fertil Steril. 2011;95(2):652-7. DOI: 10.1016/j.fertnstert.2010.08.019
17. Simon L, Liu L, Murphy K, Ge S, Hotaling J, Aston K, et al. Comparative analysis of three sperm DNA damage assays and sperm nuclear protein content in couples undergoing assisted reproduction treatment. Hum Reprod. 2014;29(5):904-17. DOI: 10.1093/humrep/deu040
18. Bungum M, Bungum L, Giwercman A. Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility. Asian J Androl. 2011;13(l):69-75.
19. Zini A, Boman J, Belzile E, Ciampi A. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis. Hum Reprod. 2008;23(12):2663-8. DOI: 10.1093/humrep/den321
20. Collins J, Barnhart K, Schlegel P. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? Fertil Steril. 2008;89:823-31.
21. Sadeghi M, Lakpour N, Heidari-Vala H, Hodjat M, Amirjannati N, Hossaini Jadda H, et al. Relationship between sperm chromatin status and ICSI outcome in men with obstructive azoospermia and unexplained infertile normozoospermia. Rom J Morphol Embryol. 2011;52(2):645-51.
22. Sharbatoghli M, Valojerdi M, Amanlou M, Khosravi F, Jafar-abadi M. Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection. Arch Gynecol Obstet. 2012;286(5):1315-22. DOI: 10.1007/s00404-012-2440-1
23. Beshay V, Bukulmez O. Sperm DNA damage: how relevant is it clinically? Curr Opin Obstet Gynecol. 2012;24(3):172-9.
24. Tavalaee M, Nasr-Esfani M. Etiology and evaluation of sperm chromatine anomalies. Int J Fertil Steril. 2008;2(1):1-8.
25. Ajina T, Ammar O, Haouas Z, Sallem A, Ezzi L, Grissa I, et al. Sakly W. Assessment of human sperm DNA integrity using two cytochemical tests: Acridine orange test and toluidine blue assay. Andrologia. 2017;49(10). DOI: 10.1111/and.12765
26. Evenson DP. The Sperm Chromatin Structure Assay (SCSA(®)) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility. Anim Reprod Sci. 2016;169:56-75. DOI: 10.1016/j.anireprosci.2016.01.017
27. Robinson L, Gallos I, Conner S, Rajkhowa M, Miller D, Lewis S, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod. 2012;27(10):2908-17. DOI: 10.1093/humrep/des261
28. Meseguer M, Santiso R, Garrido N, García-Herrero S, Remohí J, Fernandez J. Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality. Fertil Steril. 2011;95(1):124-8. DOI: 10.1016/j.fertnstert.2010.05.055
29. Roque M, Esteves S. Effect of varicocele repair on sperm DNA fragmentation: a review. Int Urol Nephrol. 2018;50(4):583-603. DOI: 10.1007/ s11255-018-1839-4
30. Sakkas D, Alvarez J. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril. 2010;93(4):1027-36.
31. Schulte R, Ohl D, Sigman M, Smith G. Sperm DNA damage in male infertility: etiologies, assays, and outcomes. J Assist Reprod Genet. 2010;27(1):3-12. DOI: 10.1007/s10815-009-9359-x
32. Aitken R, Findlay J, Hutt K, Kerr J. Apoptosis in the germ line. Reproduction. 2011;141(2):139-50. DOI: 10.1530/REP-10-0232
33. Aitken R, Krausz C. Oxidative stress, DNA damage and the Y chromosome. Reproduction. 2001;122:497-506.
34. Oosterhuis G, Mulder A, Kalsbeek-Batenburg E, Lambalk C, Schoemaker J, Vermes I. Measuring apoptosis in human spermatozoa: a biological assay for semen quality? Fertil Steril. 2000;74(2):245-50.
35. Karabulut S, Demiroğlu-Zergeroğlu A, Yılmaz E, Kutlu P, Keskin İ. Effects of human sperm cryopreservation on apoptotic markers in normozoospermic and non-normozoospermic patients. Zygote. 2018;26(4):308-13. DOI: 10.1017/S0967199418000254
36. Cohen-Bacrie P, Belloc S, Ménézo YJ, Clement P, Hamidi J, Benkhalifa M. Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients. Fertil Steril. 2009;91(5):1801-5. DOI: 10.1016/j.fertnstert.2008.01.086
37. Saylan A, Erimsah S. High quality human sperm selection for IVF: A study on sperm chromatin condensation. Acta Histochem. 2019;121(7):798- 803. DOI: 10.1016/j.acthis.2019.07.006
38. Benchaib M, Braun V, Lornage J, Hadj S, Salle B, Lejeune H, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod. 2003;18(5):1023-8.
39. Muratori M, Marchiani S, Tamburrino L, Baldi E. Sperm DNA Fragmentation: Mechanisms of Origin. Adv Exp Med Biol. 2019;1166:75-85. DOI: 10.1007/978-3-030-21664-1_5
40. Salsabili N, Mehrsai A, Jalalizadeh B, Pourmand G, Jalaie S. Correlation of sperm nuclear chromatin condensation staining method with semen parameters and sperm functional tests in patients with spinal cord injury, varicocele, and idiopathic infertility. Urol J. 2006;3(1):32-7.
41. Chi H, Chung D, Choi S, Kim J, Kim G, Lee J, et al. Integrity of human sperm DNA assessed by the neutral comet assay and its relationship to semen parameters and clinical outcomes for the IVF-ET program. Clin Exp Reprod Med. 2011;38(1):10-7. DOI: 10.5653/cerm.2011.38.1.10
42. Avendaño C, Franchi A, Duran H, Oehninger S. DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome. Fertil Steril. 2010;94(2):549-57. DOI: 10.1016/j.fertnstert.2009.02.050
43. Esteves S, Roque M, Bradley C, Garrido N. Reproductive outcomes of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with high levels of DNA fragmentation in semen: systematic review and meta-analysis. Fertil Steril. 2017;108(3):456-67. DOI: 10.1016/j.fertnstert.2017.06.018
44. Seli E, Sakkas D. Spermatozoal nuclear determinants of reproductive outcome: implications for ART. Hum Reprod Update. 2005;11(4):337-49.
45. Delbes G, Hales B, Robaire B. Toxicants and human sperm chromatin integrity. Mol Hum Reprod. 2010;16(1):14-22.
46. Tu H, Zini A. Finasteride-induced secondary infertility associated with sperm DNA damage. Fertil Steril. 2011;95(6):2125:13-4.
47. Abdelbaki S, Sabry J, Al-Adl A, Sabry H. The impact of coexisting sperm DNA fragmentation and seminal oxidative stress on the outcome of varicocelectomy in infertile patients: A prospective controlled study. Arab J Urol. 2017;15(2):131-9. DOI: 10.1016/j.aju.2017.03.002
48. Tremellen K. Oxidative stress and male infertility – a clinical perspective. Hum Reprod Update. 2008;14:243-58.
49. Zini A, Al-Hathal N. Antioxidant therapy in male infertility: fact or fiction? Asian J Androl. 2011;13(3):374-81.
50. Showell M, Mackenzie-Proctor R, Brown J, Yazdani A, Stankiewicz M, Hart R. Antioxidants for male subfertility. Cochrane Database Syst Rev. 2014;12:CD007411. DOI: 10.1002/14651858.CD007411.pub3
51. Bozhedomov VA, Gromenko DS, Ushakova IV. Oksidativnyiy stress spermatozoidov v patogeneze muzhskogo besplodiya. Urologiya. 2009;2:51- 6. [in Russian].

«Вістник проблем біології і медицини» Випуск 4 Том 1 (153), 2019 рік , 31-34 сторінки, код УДК 616.699-07:577.2.088.7

10.29254/2077-4214-2019-4-1-153-31-34